Agilent 1260 infinity ii инструкция на русском

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  Agilent InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Agilent Technologies…
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  Use, duplication or disclosure of Software is subject to Agi- lent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
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  In This Guide… In This Guide… This manual covers the Agilent InfinityLab LC Series 1260 Infinity II Binary Pump (G7112B). 1 Introduction This chapter gives an introduction to the module and an instrument overview. 2 Site Requirements and Specifications This chapter provides information about site requirements and specifications for the binary pump.
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  This chapter provides detailed technical information about your binary pump. 12 LAN Configuration This chapter provides information on connecting the module to the Agilent ChemStation PC. 13 Appendix This chapter provides addition information on safety, legal and web. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 5: Table Of Contents

  When to Use a Vacuum Degasser When to Use the Active Seal Wash Option When to Use the Low Volume Mixer When to Remove Damper and Mixer How to Optimize the Compressibility Compensation Setting InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

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  Exchange the Seal Wash Cartridge Replace Leak Handling System Parts Exchange the Outlet Valve Installation of the Solvent Selection Valve Upgrade Kit Exchange the Solvent Selection Valve Replacing Module Firmware InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
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  Setting the 6-bit Configuration Switch Early Maintenance Feedback Instrument Layout 12 LAN Configuration What You Have to Do First TCP/IP parameter configuration Configuration Switches Initialization Mode Selection Dynamic Host Configuration Protocol (DHCP) Manual Configuration InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
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  Contents 13 Appendix General Safety Information Waste Electrical and Electronic Equipment (WEE) Directive Radio Interference Sound Emission Agilent Technologies on the Internet InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 9: Introduction

  InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Introduction Instrument and Operation Introduction to the Pump Principle of Operation Product Description Features Overview of the Hydraulic Path Leak and Waste Handling Leak Sensor Waste Concept This chapter gives an introduction to the module and an instrument overview.

• Page 10: Instrument And Operation

  (optional) allows to form binary mixtures (isocratic or gradient) from one of two solvents per channel. Active seal wash (optional) is available for use with concentrated buffer solutions. Figure 1 Overview of the binary pump InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 11: Principle Of Operation

  A pressure sensor monitors the pump pressure. A purge valve with integrated PTFE frit is fitted to the pump outlet for convenient priming of the pumping system. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

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  64. Bypassing the mixer while the damper remains in line is not a supported configuration and N O T E may lead to undesired behavior of the binary pump. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
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  Introduction Instrument and Operation Figure 3 The hydraulic path of the Binary Pump with bypassed damper and mixer For pump specifications, see “Performance Specifications” on page 30. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 14: Product Description

  Introduction Instrument and Operation Product Description The Agilent 1260 Infinity II Binary Pump is suited for LC applications using conventional or superficially porous particles columns, as well as STM column technology. Gradient formation is based on a high-pressure mixing principle.

• Page 15: Overview Of The Hydraulic Path

  The solvent enters the pump heads close to the bottom limit and leaves it at its top. The outer diameter of the piston is smaller than InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

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  During the drawing stroke of the first piston, the second piston delivers the drawn volume into the system. For pump specifications, see “Performance Specifications” on page 30. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
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  Solvent compressibility calibrations acquired with a miscalibrated pump will work, but they are not transferable to other pumps. A correct pump elasticity calibration is an essential prerequisite for successful solvent compressibility calibrations. ➔ Calibrate the pump elasticity correctly. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
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  Unfortunately, the influence of the temperature on compressibility is non-linear and cannot be calculated. The Agilent 1260 Infinity II Binary Pump features a multi point compressibility calibration. The compressibility of a solvent is determined at different pressures from 0 –…
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  The stroke volume for the pump is by default set to AUTO mode. This means that the stroke is optimized for the flow rate in use. A change to larger stroke volumes is possible but not recommended. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 20: Leak And Waste Handling

  • from the Multisampler needle wash port • from the Sample Cooler or Sample Thermostat (condensate) • from the pump’s Seal Wash Sensor (if applicable) • from the pump’s Purge Valve or Multipurpose Valve InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

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  Introduction Leak and Waste Handling Figure 6 Infinity II Leak Waste Concept (Flex Bench installation) InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
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  Introduction Leak and Waste Handling Figure 7 Infinity II Single Stack Leak Waste Concept (bench installation) InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 23: Leak Sensor

  The solvent DMF (dimethyl formamide) leads to corrosion of the leak sensor. The material of the leak sensor, PVDF (polyvinylidene fluoride), is incompatible with DMF. ➔ Do not use DMF. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 24: Waste Concept

  1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don’t immerse in the liquid. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 25: Site Requirements And Specifications

  InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications This chapter provides information about site requirements and specifications for the binary pump. Agilent Technologies…

• Page 26: Site Requirements

  ➔ Make sure the power connector of the instrument can be easily reached and unplugged. ➔ Provide sufficient space behind the power socket of the instrument to unplug the cable. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 27: Site Requirements

  Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Absence of ground connection WA R N I N G The absence of ground connection can lead to electric shock or short circuit.

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  ➔ If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 29: Physical Specifications

  For storing the module Safety standards: Installation category II, Pollution degree 2 For indoor use only. IEC, EN, CSA, UL ISM Classification ISM Group 1 Class B According to CISPR 11 InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 30: Performance Specifications

  Standard delay volume configuration: Delay volume measured with water at 600 – 900 µL, (includes 400 µL mixer), 1 mL/min (water/water with dependent on back pressure tracer) Low delay volume configuration:120 µL InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

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  Electronic records of maintenance and errors. Housing All materials are recyclable InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
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  Site Requirements and Specifications Performance Specifications InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 33: Using The Pump

  InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Using the Pump Magnets Turn on/off Status Indicators Best Practices Daily / Weekly tasks Power up / Shut-down the pump Prepare the pump How to deal with solvents Leak and Waste Handling…

• Page 34: Magnets

  Using the Pump Magnets Magnets This stack exemplarily shows the magnets’ positions in the modules. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 35: Turn On/Off

  Using the Pump Turn on/off Turn on/off Power switch: On Turn instrument On/Off with the control software. Power switch: Off InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

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  Using the Pump Turn on/off InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 37: Status Indicators

  5. Resident mode (blinking) — for example during update of main firmware. 6. Bootloader mode (fast blinking). Try to re-boot the module or try a cold-start. Then try a firmware update. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 38: Best Practices

  • Flush system with water to remove buffer. • Remove all samples from the sampler and store according to good laboratory practice. • Use recommended solvents to store the system. • Power off the system. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 39: Prepare The Pump

  • Label bottles correctly with bottle content, and filling date / expiry date. • Use solvent inlet filters. • Reduce risk of algae growth: use brown bottles for aqueous solvents, avoid direct sunlight. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 40: Leak And Waste Handling

  N O T E has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet. For details on correct installation, see separate installation documentation. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 41: Hints For Successful Use Of The Binary Pump

  • After changing the piston seals, apply the seal wear-in procedure (see “Seal Wear-in Procedure” on page 136). • Place the aqueous solvent on channel A and the organic solvent on channel B. The default compressibility settings are set accordingly. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 42: Setting Up The Pump With The G4208A Instant Pilot

  Pilot G4208A User’s Guide (G4208-90006). Details about setting up module specific parameters can be found in the Instant Pilot online help. The pump parameters are described in detail in “Overview” on page 43. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 43: Setting Up The Pump With The Instrument Control Interface

  Parameters described in following sections are offered by the instrument control interface and can usually be accessed through Agilent instrument control software. For details, please refer to manuals and online help of respective user interfaces. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 44: Setup Of Basic Pump Parameters

  • Display There are three ways to display the timetable: • in tabular form • as flow/pressure graph • as solvent percentage plot Values can only be changed in tabular view. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 45: Pump Control

  C AU T I O N This can result in a rapid and uncontrolled pressure increase. ➔ To prevent harm to the column, open the purge valve until the initialization is finished. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 46: Data Curves

  N O T E and flow are calculated from the method settings of the pump. For details, please refer to the online help or manual of your instrument control software. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 47: Bottle Filling

  If this option is checked, the pump will turn off before air is aspirated. However, off… the residual solvent volume has been calculated for 1 L solvent bottles and may be too small for large bottles or other vessels. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 48: Solvent Information

  Pump head (body) Pistons Sapphire Piston seals/wash seals PTFE, SST (reversed phase) or UHMW-PE, SST (normal phase) Pressure sensor Purge valve SST, gold, PTFE, ceramic Damping unit SST, gold Capillaries/fittings Tubings PTFE InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

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  PEEK capillaries keeps the flow path free of steel and ensures pressure stability to at least 600 bar. If in doubt, consult the available literature about the chemical compatibility of PEEK. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
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  It can be corroded by strong bases (e.g. hydroxide solutions > 10 %, diethylamine). It is not recommended for the use with fluoric acid and fluorides. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
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  For example, a 1 % solution of acetic acid in methanol will attack steel. • Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid). • Mixtures of carbon tetrachloride with 2-propanol or THF. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
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  It can be corroded by complexing cyanides and concentrated acids like aqua regia. Zirconium Oxide (ZrO Zirconium Oxide is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
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  Sapphire, Ruby and Al -based ceramics Sapphire, ruby and ceramics based on aluminum oxide Al are inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 54: Algae Growth In Hplc Systems

  • Use the amber solvent bottle ( Solvent bottle, amber, 1000 mL (9301-6526)) supplied with the instrument for your aqueous mobile phase. • If possible add a few mg/l sodium azide or a few percent organic solvent to the aqueous mobile phase. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 55: Prevent Blocking Of Solvent Filters

  Observe appropriate safety procedures (for example, wear goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the solvent vendor, especially when toxic or hazardous solvents are used. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 56: Cleaning The Solvent Filters

  • Thoroughly flush the filter with HPLC-grade water (remove all nitric acid, some capillary columns can be damaged by nitric acid). • Replace the filter. Never use the system without solvent filter installed. N O T E InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 57: Normal Phase Applications

  Do not use normal phase valves in applications running with aqueous solutions. The N-Valves have been tested successfully in using hexane at pressures below 100 bar; heptane can be used as a substitute for neurotoxic hexane. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

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  Type N (G4204-60122) Outlet valves 1260 Infinity Outlet Valve Type 1290 Infinity Outlet Valve Type N/SFC (G1312-60167) N (G4220-60128) Seals PE seals (pack of 2) (0905-1420) Wash Seal PE (0905-1718) InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 59: Optimizing Performance

  InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Optimizing Performance When to Use a Vacuum Degasser Operational Hints for the Vacuum Degasser When to Use the Active Seal Wash Option When to Use the Low Volume Mixer When to Remove Damper and Mixer…

• Page 60: When To Use A Vacuum Degasser

  0.5 mL/min). • The binary pump is used with bypassed damper and mixer. The external 1260 Infinity II Degasser G7122A is recommended for use with applications using highly volatile solvents like Hexane or DCM, solvents with special characteristics like THF, or applications using refractive index detection.

• Page 61: When To Use The Active Seal Wash Option

  The operation of the peristaltic pump can be controlled from the data system or the Instant Pilot. For adding a seal-wash option, please contact your local Agilent Technologies service representative. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 62: When To Use The Low Volume Mixer

  The Low volume mixer ( 200 μL) (5067-1565) is designed for use with the Agilent InfinityLab LC Series 1260 Infinity II Binary LC System in low delay volume mode. This configuration is typically used for 2.1 mm i.d., 1.8 μm particle size columns, where emphasis is put on S/N ratio.

• Page 63: When To Remove Damper And Mixer

  For applications that require lowest delay volume (for example, fast gradient methods or gradient applications with low flow rates), damper and mixer can be bypassed. Figure 9 Flow path modifications of the Binary Pump InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 64: Convert The Binary Pump To Low Delay Volume Mode

  This paragraph shows how to bypass damper and mixer and convert the pump to low delay volume mode. Configurations where only damper or mixer are disconnected while the other part is still in line are not supported by Agilent Technologies. Tools required Description…

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  Disconnect fitting A from outlet 1 of the mixer. Connect fitting A to port 2 of the pressure sensor. Seal port 1 of the mixer with a plastic blank nut. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 66: How To Optimize The Compressibility Compensation Setting

  400 bar binary pump, an average compressibility value for the solvent is sufficient. For the 600 bar 1260 Infinity II Binary Pump , the pressure-dependency of a solvent compressibility needs to be considered. It is determined at different pressures between 0 – 600 bar. The pump uses the obtained non-linear function to select the correct compressibility value for the actual pump pressure.

• Page 67: Optimization Of Legacy Compressibility Settings

  10. The compressibility compensation setting that generates the smallest pressure ripple is the optimum value for your solvent composition. 6 Repeat step 1 through step 5 for the B channel of your binary pump. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

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  How to Optimize the Compressibility Compensation Setting Table 8 Solvent Compressibility Solvent (pure) Compressibility (10 /bar) Acetone Acetonitrile Benzene Carbon tetrachloride Chloroform Cyclohexane Ethanol Ethyl acetate Heptane Hexane Isobutanol Isopropanol Methanol 1-Propanol Toluene Water InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 69: Troubleshooting And Diagnostics

  InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions User Interfaces Agilent Lab Advisor Software Overview of the troubleshooting and diagnostic features. Agilent Technologies…

• Page 70: Overview Of The Module’s Indicators And Test Functions

  (i.e. the high pressure flow path between pump and column). After exchanging flow path components (e.g. pump seals or injection seal), use this test to verify the system is pressure tight, see “(System) Pressure Test” page 96. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

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  When a problem with the pump is suspected, use this test to help troubleshoot the pump and its pumping performance, see “Pump Leak Rate Test” on page 107. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 72: User Interfaces

  Depending on the user interface, the available tests vary. Some descriptions are only available in the Service Manual. Test Instant Pilot G4208A Agilent Lab Advisor Pressure Test Valve Test Solvent compressibility calibration Pump elasticity calibration InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 73: Agilent Lab Advisor Software

  The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual. For details, refer to the Agilent Lab Advisor software help files. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

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  Troubleshooting and Diagnostics Agilent Lab Advisor Software InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 75: Error Information

  InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Error Information What Are Error Messages General Error Messages Timeout Shutdown Remote Timeout Lost CAN Partner Leak Leak Sensor Open Leak Sensor Short Compensation Sensor Open Compensation Sensor Short Fan Failed…

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  Degasser: Vacuum cannot be maintained Degasser: limit not reached This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 77: What Are Error Messages

  In all cases, error propagation is done via the CAN bus or via an APG/ERI remote cable (see documentation for the APG/ERI interface). InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 78: General Error Messages

  Check the vacuum degasser for an error The degasser failed to generate sufficient condition. Refer to the Service Manual for the vacuum for solvent degassing. degasser or the pump that has the degasser built-in. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 79: Remote Timeout

  Exchange the CAN cable. Defective CAN cable. Switch off the system. Restart the system, and Defective main board in another module. determine which module or modules are not recognized by the system. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 80: Leak

  If there are still signs of a leak, exchange the valve, or outlet valve. appropriate seal (purge valve, active inlet valve, outlet valve). Exchange the pump seals. Defective pump seals. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 81: Leak Sensor Open

  Leak sensor incorrectly routed, being pinched by a metal component. Please contact your Agilent service representative. Power switch assembly defective Please contact your Agilent service representative. Cable or contact problem. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 82: Compensation Sensor Open

  Probable cause Suggested actions Please contact your Agilent service Defective power switch assembly representative. Please contact your Agilent service Loose connection between the power representative. switch board and the main board InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 83: Fan Failed

  Please contact your Agilent service Defective fan. representative. Please contact your Agilent service Defective main board. representative. Please contact your Agilent service Improperly positioned cables or wires representative. obstructing fan blades. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 84: Module Error Messages

  (autosampler), sample loop (autosampler), column frits and capillaries with small internal diameters (e.g. 50 µm ID). Please contact your Agilent service representative. Defective pressure sensor. Please contact your Agilent service representative. Defective main board. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 85: Pressure Below Lower Limit

  -120 mV across the pressure sensor. Probable cause Suggested actions Please contact your Agilent service representative. Pressure sensor disconnected Please contact your Agilent service representative. Defective pressure sensor InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 86: Valve Failed

  Please contact your Agilent service Connection cable (inside instrument) not representative. connected. Please contact your Agilent service Connection cable (inside instrument) representative. defective. Exchange the solvent selection valve. Solvent selection valve defective. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 87: Electronic Fuse Of Ssv Open

  Please contact your Agilent service Defective connection cable (front panel to representative. main board). Please contact your Agilent service Defective main board. representative. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 88: Motor-Drive Power

  Please contact your Agilent service Defective pump drive assembly. representative. Please contact your Agilent service Defective main board. representative. Exchange restriction capillary. Restriction capillary blocked at pre-mixing union. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 89: Encoder Missing

  Probable cause Suggested actions Please contact your Agilent service Defective or disconnected pump encoder representative. connector. Please contact your Agilent service Defective pump drive assembly. representative. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 90: Servo Restart Failed

  Exchange the (passive or active) inlet valve. Blocked (passive or active) inlet valve. Please contact your Agilent service Defective pump drive assembly. representative. Please contact your Agilent service Defective main board. representative. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 91: Pump Head Missing

  Irregular or sticking drive movement. pistons, and internal components for signs of wear, contamination or damage. Exchange components as required. Please contact your Agilent service Defective pump drive assembly. representative. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 92: Index Adjustment

  Probable cause Suggested actions Please contact your Agilent service representative. Disconnected or defective encoder cable. Please contact your Agilent service representative. Defective pump drive assembly. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 93: Initialization Failed

  • Measured pressure is higher than 32000 hPa Probable cause Suggested actions Please contact your Agilent service Degasser sensor defect representative. Please contact your Agilent service Degasser sensor not connected to main representative. board. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 94
  Please contact your Agilent service Liquid in degasser tubing. representative. Please contact your Agilent service Leak in degasser tubing or chamber. representative. Please contact your Agilent service Degasser vacuum pump defect. representative. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 95: Test Functions And Calibration

  InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Test Functions and Calibration (System) Pressure Test Positioning the Blank Nut Running the System Pressure Test Evaluating the Results Valve Test Running the Valve Test Evaluating the Results Binary Pump Solvent Compressibility Calibration…

• Page 96: (System) Pressure Test

  When the system pressure reaches 600 bar, the pump continues to pump at a flow rate that keeps the pressure constant. The flow that is needed to keep the pressure constant is directly translated into a leak rate. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 97: Positioning The Blank Nut

  Pressure Test again. If the test passes, the defective component is located after the blank nut. Confirm the diagnosis by placing the blank nut immediately after the suspected component. The diagnosis is confirmed if the test fails. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 98: Running The System Pressure Test

  N O T E is caused by the abnormal termination during the operation of the test whereas a failure of a test indicates that the test results were not within the specified limits. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 99
  Tighten the fitting or exchange the capillary. Damaged pump seals or pistons. Run the valve test to identify the defective component. Loose purge valve. Tighten the purge valve nut (14 mm wrench). InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 100
  Potential Cause (Column Compartment) Potential Cause (Column Compartment) Corrective Action Loose or leaky fitting. Tighten or exchange the fitting or capillary. Rotor seal (column switching valve). Exchange the rotor seal. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 101: Valve Test

  The procedure from step 1 is repeated on pump head B. Step 3 The data from step 1 and 2 are evaluated. In case test failed, a conclusion about the defective part is made. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 102: Running The Valve Test

  Make sure to release the pressure by opening the purge valve when the test has N O T E completed. Otherwise the pump may generate an overpressure error. Evaluating the Results Refer to the help file of the Agilent Lab Advisor for further details. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 103: Binary Pump Solvent Compressibility Calibration

  The compressibility data set for this solvent is stored in an XML-file on the desktop. It can be shared with other InfinityLab LC Series binary pumps via the controlling data system. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 104: Running The Solvent Compressibility Calibration

  2 Start the test and follow the instructions. Make sure to release the pressure by opening the purge valve when the test has N O T E completed. Otherwise the pump may generate an overpressure error. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 105: Pump Elasticity Calibration

  Solvent compressibility calibrations acquired with a miscalibrated pump will work, but they are not transferable to other pumps. A correct pump elasticity calibration is an essential prerequisite for successful solvent compressibility calibrations. ➔ Calibrate the pump elasticity correctly. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 106: Running The Pump Elasticity Calibration

  2 Start the test and follow the instructions. Make sure to release the pressure by opening the purge valve when the test has N O T E completed. Otherwise the pump may generate an overpressure error. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 107: Pump Leak Rate Test

  1 Select the Pump Leak Rate Test from the Test Selection menu. 2 Start the test and follow the instructions. Make sure to release the pressure by slowly opening the purge valve when the test has N O T E finished. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 108: Evaluating The Results

  Results of the leak rate test are the leak rates measured for pistons 1 and 2 as described for the test principle. If any of the leak rates exceeds 3 μL/min, the test will fail. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 109: Potential Causes Of Leak Rate Test Failure

  Suggested actions Inspect piston, replace piston and/or seal Leak at piston 1 or seal in chamber 1 Replace inlet valve or inlet valve cartridge (AIV Leak at inlet valve only) InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 110
  Probable cause Suggested actions Replace the part which has failed and re-run Leak at outlet valve the test. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 111: Maintenance

  InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Maintenance Introduction to Maintenance Cautions and Warnings Cleaning the Module Overview of Maintenance and Simple Repairs Maintenance Procedures Remove and Install Doors Exchange the Purge Valve Frit or the Purge Valve…

• Page 112: Introduction To Maintenance

  These procedures are described in “Overview of Maintenance and Simple Repairs” on page 116. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 113: Cautions And Warnings

  ➔ The volume of substances should be reduced to the minimum required for the analysis. ➔ Do not operate the instrument in an explosive atmosphere. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 114
  If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 115: Cleaning The Module

  WA R N I N G hazard and damage the module ➔ Do not use an excessively damp cloth during cleaning. ➔ Drain all solvent lines before opening any connections in the flow path. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 116: Overview Of Maintenance And Simple Repairs

  “Exchange the Active Inlet Valve (AIV) or its Cartridge” page 137 Pump head, see “Maintenance of a Pump Head with Seal Wash” on page 130 Solvent selection valve, see “Exchange the Solvent Selection Valve” on page 148 InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 117: Maintenance Procedures

  Pressure ripple unstable, run Pump Leak Rate Test for verification “Exchange the Solvent Selection Valve” If internally leaking Cross port flow page 148 If solenoid is defective Error message “Valve Failed” InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 118: Remove And Install Doors

  The figures shown in this procedure exemplarily show the Infinity II Multisampler module. N O T E The principle of how to remove and/or install doors works in the same way for all Infinity II modules. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 119
  Press the release buttons and pull the front door out. For the Installation of the front door. Insert the hinges into their guides and move the door in until the release buttons click into their final position. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 120: Exchange The Purge Valve Frit Or The Purge Valve

  Using the 14 mm wrench, unscrew the purge valve and capillary from the purge valve. Disconnect the waste remove it from the purge valve holder. tube. Beware of leaking solvents due to hydrostatic pressure. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 121
  (slit in frit points to the front). N O T E Before reinstallation always check the gold seal in the seal cap. A deformed seal cap should be exchanged. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 122
  The rubber o-ring might break. ➔ Anticipate the correct position of the connections before tightening the valve. Tighten the purge valve and reconnect outlet capillary and waste tubing. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 123: Replace The O-Ring On The Purge Valve

  • Use an optional solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding leakages. • Remove the purge valve from the pump head. Disassemble the purge valve. Remove the old o-ring from the purge valve. Clean the purge valve parts. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 124
  Reinstall the seal cap including the gold seal. pump. The o-ring will take damage. N O T E Before reinstallation always check the gold seal in the seal cap. A deformed seal cap should be exchanged. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 125: Remove The Pump Head Assembly

  The following procedure describes the removal and disassembly of pump head A (left). For pump head B (right) proceed in the same way and skip steps that deal with the purge valve. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 126
  Using a 4 mm hexagonal key stepwise loosen and Unplug the active inlet valve cable from the connector. remove the two pump head screws. Remove the pump head assembly from the module. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 127: Maintenance Of A Pump Head Without Seal Wash

  The following procedure describes the removal and disassembly of pump head A (left). For pump head B (right) proceed in the same way and skip steps that deal with the purge valve. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 128
  Using the steel side of the insert tool, carefully remove layers: clean the piston surface with abrasive paper and the seal from the pump housing. rinse with 2-propanol. Replace piston if scratched. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 129
  Place the pump housing onto the piston housing. Insert the pistons and carefully press them into the seals. Tighten the lock screw. Install the pump head, see “Reinstall the Pump Head Assembly” on page 134. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 130: Maintenance Of A Pump Head With Seal Wash

  The following procedure describes the removal and disassembly of pump head A (left). For pump head B (right) proceed in the same way and skip steps that deal with the purge valve. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 131
  Lift the housing away from the pistons. Check the piston surface and remove any deposits or layers: clean the piston surface with abrasive paper and rinse with 2-propanol. Replace piston if scratched. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 132
  Place the support rings on the piston housing. Note the ring. Use a matching orientation of gasket and support correct position of the pins on the support ring. ring. Put the seal holder on top of the gasket. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 133
  Place the pump housing onto the piston housing. Insert the pistons and carefully press them into the seals. Tighten the lock screw. Install the pump head, see “Reinstall the Pump Head Assembly” on page 134. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 134: Reinstall The Pump Head Assembly

  Slide the pump head assembly onto the pump drive. Using a 4 mm hexagonal key, tighten the pump head Reconnect the active inlet valve cable to the connector. screws stepwise with increasing torque. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 135
  Maintenance Reinstall the Pump Head Assembly Reconnect all tubings and capillaries. Close the doors. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 136: Seal Wear-In Procedure

  Reconnect the intake tubing to the solvent selection valve and the connecting tube from the solvent selection valve (if installed) to the AIV. 7 Purge your system with the solvent used for your next application. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 137: Exchange The Active Inlet Valve (Aiv) Or Its Cartridge

  C AU T I O N Overtightening will destroy the active inlet valve cartridge. ➔ Tighten the active inlet valve properly. Open the doors. Unplug the active inlet valve cable from the connector. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 138
  Screw the new valve into the pump head. With the Position the valve so that the solvent inlet tube 14 mm wrench, turn the nut until it is hand tight. connection points towards the front. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 139
  N O T E in the current application before the flow stabilizes at a pressure ripple as low as it used to be when the system was still working properly. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 140: Exchange The Seal Wash Cartridge

  Remove the wash solvent tubings from the support ring Unclip the peristaltic pump cartridge from the module outlet and from the adapter leading to the waste bottle. housing and remove it. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 141
  Connect the peristaltic pump tubes to the support rings the pump motor and push the plastic clips into the outlet and to the adapter leading to the waste bottle. module housing. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 142: Replace Leak Handling System Parts

  (2.). Install the Leak Adapter by pressing it into the Main Insert the Tubing (approximately 85 mm required for Cover. replacement) between Leak Adapter outlet and Leak Panel. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 143
  Maintenance Replace Leak Handling System Parts Close the doors. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 144: Exchange The Outlet Valve

  Unscrew the valve with the 14 mm wrench and remove it from the outlet valve. from the pump body. N O T E Do not disassemble the outlet valve, as this can damage the valve. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 145
  Maintenance Exchange the Outlet Valve Reinstall the outlet valve and tighten it using a torque Reconnect the capillary. wrench (approx.12 Nm). InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 146: Installation Of The Solvent Selection Valve Upgrade Kit

  Guide the SSV cable through the slots in the distance connection socket inside (behind the pump front metal sheet and route it through the slot in the pump front panel). metal panel to the internal connection socket. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 147
  N O T E mL of solvent to get the flow stabilized at a pressure ripple as low as it used to be when the system was still working properly. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 148: Exchange The Solvent Selection Valve

  Place the bottles back into the solvent cabinet. Using a screwdriver, loosen the holding screws of the Carefully pull the valve holder out. valve holder. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 149
  Bend the cable at the connector of the new valve. Guide the cable and connector into the hole and push the connector into the socket. Exchange the defective solvent selection valve. Tighten the screws of the valve holder. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 150
  After an exchange of the valve it may be required to pump several mL of solvent before the N O T E flow stabilizes at a pressure ripple as low as it used to be when the system was still working properly. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 151: Replacing Module Firmware

  Agilent web. http://www.agilent.com/en-us/firmwareDownload?whid=69761 2 For loading the firmware into the module follow the instructions in the documentation. Module Specific Information There is no specific information for this module. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 152
  Maintenance Replacing Module Firmware InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 153: Parts And Materials For Maintenance

  InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Parts and Materials for Maintenance Hydraulic Path with Solvent Selection Valve Hydraulic Path without Solvent Selection Valve Pump Head Assembly Without Seal Wash Pump Head Assembly with Seal Wash Option…

• Page 154: Hydraulic Path With Solvent Selection Valve

  Capillary ST 0.17 mm x 900 mm SI/SX ps-ps 5064-5444 Peristaltic pump cartridge, silicone tubing 5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m for seal wash option 5062-2461 Waste tube, 5 m (reorder pack) InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 155
  Parts and Materials for Maintenance Hydraulic Path with Solvent Selection Valve Figure 11 Hydraulic Path with Solvent Selection Valve InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 156: Hydraulic Path Without Solvent Selection Valve

  Capillary ST 0.17 mm x 900 mm SI/SX ps-ps 5065-4445 Peristaltic pump with Pharmed tubing 5062-2461 Waste tube, 5 m (reorder pack) 5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m for seal wash option InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 157
  Parts and Materials for Maintenance Hydraulic Path without Solvent Selection Valve Figure 12 Hydraulic Path without Solvent Selection Valve, with Active Seal Wash InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 158: Pump Head Assembly Without Seal Wash

  Lock screw 12 a G1312-60025 Active inlet valve body, without cartridge 12 b G1312-60020 Cartridge for active inlet valve 600 bar G1312-23201 Adapter 0515-2118 Pump head screw (M5, 60 mm) InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 159
  Parts and Materials for Maintenance Pump Head Assembly Without Seal Wash Figure 13 Pump Head Assembly Without Seal Wash InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 160: Pump Head Assembly With Seal Wash Option

  Lock screw G1311-25200 Pump chamber housing G1312-60025 Active inlet valve body, without cartridge G1312-60020 Cartridge for active inlet valve 600 bar G1312-23201 Adapter 0515-2118 Pump head screw (M5, 60 mm) InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 161
  Parts and Materials for Maintenance Pump Head Assembly with Seal Wash Option Figure 14 Pump Head Assembly with Seal Wash Option InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 162: Outlet Valve

  Parts and Materials for Maintenance Outlet Valve Outlet Valve Description G1312-60067 Outlet valve Figure 15 Outlet valve InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 163: Purge Valve Assembly

  Purge Valve Assembly Purge Valve Assembly Item Description G7111-60061 Purge valve 01018-22707 PTFE frits (pack of 5) 5067-4728 Seal cap 5067-6595 1260 PV O-ring FKM 5/pack Figure 16 Purge Valve Assembly InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 164: Active Inlet Valve Assembly

  Active Inlet Valve Assembly Active Inlet Valve Assembly Item Description G1312-60025 Active inlet valve body, without cartridge G1312-60020 Cartridge for active inlet valve 600 bar Figure 17 Active Inlet Valve Assembly InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 165: Hplc System Tool Kit

  Parts and Materials for Maintenance HPLC System Tool Kit HPLC System Tool Kit InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 166: Active Seal Wash Option

  Shock mount 1540-0455 Edge protector 5041-2120 Folding box 5065-4445 Peristaltic pump with Pharmed tubing 5042-6422 Tubing connector, 1 mm o.d. 5065-9943 Stepper Motor for the Peristaltic Pump G3010-01203 RFI Strip 10×30 InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 167: Solvent Cabinet

  Parts and Materials for Maintenance Solvent Cabinet Solvent Cabinet Description 5067-5760 Solvent Cabinet Kit For details refer to: Usage Guideline for the Solvent Cabinet (01200-90150) InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 168: Bottle Head Assembly

  5063-6599 Tube screw (10/Pk) Wire marker 5062-2483 Tube PTFE 1.5 mm x 5 m, 3 mm od 5062-8517 Inlet filter adapter (4/Pk) 5041-2168 Solvent inlet filter, 20 µm pore size InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 169: Cover Parts

  Door Assembly Infinity 180 Left 5043-1354 Name Plate 1290 Infinity 2 G7104-68713 Cabinet Kit 180 Infinity II (includes sides, bottom, top, leak adapter top and Status Indicator Insert) Figure 18 Cover Parts InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 170: Accessory Kit

  Algae note 5500-1169 Y Tube Connector ID 6.4 5500-1155 Tube Connector, 90 degree, ID 6.4 5043-1372 Tubing Connector Leak 3-1 5043-1373 Tubing Connector Leak Cap 0890-1195 PTFE tubing, 0.052 in i.d. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 171: Identifying Cables

  InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cable Kit Agilent 1200 Module to Printer This chapter provides information on cables used with the Agilent InfinityLab LC Series modules.

• Page 172: Cable Overview

  Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter…

• Page 173
  1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m USB cables Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 174: Analog Cables

  The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent Signal Name module Not connected Shield Analog — Center Analog + InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 175
  Signal Name module Shield Shield Analog — Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog — Analog + InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 176: Remote Cables

  NOT USED pink SHUT DOWN blue START PREPARE black 1wire DATA violet DGND grey-pink +5V ERI out red-blue PGND white-green PGND brown-green +24V ERI out white-yellow +24V ERI out yellow-brown InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 177
  • 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 178
  Ground Cable Shielding One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 179
  Pin Agilent Signal Name Active module (TTL) White Digital ground Brown Prepare run Gray Start Blue Shut down Pink connected Yellow Power on High Ready High Green Stop Black Start request InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 180: Can/Lan Cables

  CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 181: Rs-232 Cable Kit

  It’s also called «Null Modem Cable» with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 182: Agilent 1200 Module To Printer

  To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 183: Hardware Information

  InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Hardware Information Firmware Description Electrical Connections Serial Number Information (ALL) Rear view of the module Interfaces Overview Interfaces ERI (Enhanced Remote Interface) USB (Universal Serial Bus) Setting the 6-bit Configuration Switch…

• Page 184: Firmware Description

  • run synchronization through APG/ERI remote, • error handling, • diagnostic functions, • or module specific functions like • internal events such as lamp control, filter movements, • raw data collection and conversion to absorbance. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 185
  Update of main system can be done in the resident system only. Update of the resident N O T E system can be done in the main system only. Main and resident firmware must be from the same set. Figure 19 Firmware Update Mechanism InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 186
  All this specific information is described in the documentation provided with the firmware update tools. The firmware update tools, firmware and documentation are available from the Agilent web. • http://www.agilent.com/en-us/firmwareDownload?whid=69761 InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 187: Electrical Connections

  There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.

• Page 188: Serial Number Information (All)

  (there can be more than one code for the same module) 00000 Serial number Rear view of the module Figure 20 Rear view of the pump – electrical connections and label InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 189: Interfaces

  Module RS-232 Analog APG (A) Special (on-board) / ERI (E) Pumps G7104A/C G7110B G7111A/B, G5654A G7112B G7120A G7161A/B Samplers G7129A/B/C G7167B/C, G5667A G7157A Detectors G7114A/B G7115A G7117A/B/C G7121A/B G7162A/B G7165A InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 190
  • RS-232C as interface to a computer • USB (Universal Series Bus) as interface to a computer • REMOTE connector as interface to other Agilent products • Analog output connector(s) for signal output InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 191: Overview Interfaces

  “USB (Universal Serial Bus)” on page 196. Analog Signal Output The analog signal output can be distributed to a recording device. For details refer to the description of the module’s main board. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 192
  Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
• Page 193
  PREPARE (L) Request to prepare for analysis (for example, calibration, detector lamp on). Receiver is any module performing pre-analysis activities. Special Interfaces There is no special interface for this module. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 194: Eri (Enhanced Remote Interface)

  This way the interface can support various additional devices like sensors, triggers (in and out) and small controllers, etc. Figure 21 Location of the ERI interface (example shows a G7114A/B VWD) InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 195
  This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI-device. The firmware can detect the connected type of device automatically and update information in the device (if required). InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 196: Usb (Universal Serial Bus)

  • Short circuit will be detected through hardware. USB (Universal Serial Bus) USB (Universal Serial Bus) — replaces RS232, supports: • a PC with control software (for example Agilent Lab Advisor) • USB Flash Disk InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 197: Setting The 6-Bit Configuration Switch

  • For specific LAN modes switches 4-5 must be set as required. • For boot resident/cold start modes switches 1+2 or 6 must be UP. Figure 22 Location of Configuration switch (example shows a G7114A/B VWD) InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 198
  When selecting mode COM, settings are stored to non-volatile memory. When selecting mode TEST, COM settings are tak- en from non-volatile memory. not assigned — Always keep these switches on position ‘0’ (off) Default IP Address is 192.168.254.11 Host Name will be the MAC address. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 199: Special Settings

  Save your methods and data before executing a forced cold start. If you use the following switch settings and power the instrument up again, it will start as described above. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 200
  Boot Main System / Keep Data Boot Resident System / Keep Data Boot Main System / Revert to Default Data Boot Resident System / Revert to Default Data Note: The setting ‘0’ (down) is essential. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 201: Early Maintenance Feedback

  EMF limits, and then reset the EMF counters to zero. The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 202: Instrument Layout

  • the plastic layers help cushion the electronic and mechanical parts from physical shock, and • the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 203
  InfinityLab LC Series 1260 Infinity II Binary Pump User Manual LAN Configuration What You Have to Do First TCP/IP parameter configuration Configuration Switches Initialization Mode Selection Dynamic Host Configuration Protocol (DHCP) General Information (DHCP) Setup (DHCP) Manual Configuration With Telnet This chapter provides information on connecting the module to the Agilent ChemStation PC.

• Page 204: Lan Configuration What You Have To Do First

  PC network card using a crossover network cable (point-to-point) or • a hub or switch using a standard LAN cable. Figure 24 Location of LAN interfaces and MAC label InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 205: Tcp/Ip Parameter Configuration

  TCP/IP parameters after power-on. The parameters may be derived non-volatile memory or initialized with known default values. The initialization mode is selected by the configuration switch, see Table 18 page 207. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 206: Configuration Switches

  The module is shipped with all switches set to OFF, as shown above. To perform any LAN configuration, SW1 and SW2 must be set to OFF. N O T E InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 207: Initialization Mode Selection

  The TCP/IP connection will be established using these parameters. The parameters were configured previously by one of the described methods. Figure 26 Using Stored (Principle) InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 208
  In the Using Default mode, the parameters stored in the memory of the module are not N O T E cleared automatically. If not changed by the user, they are still available, when switching back to the mode Using Stored. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 209: Dynamic Host Configuration Protocol (Dhcp)

  2 It may be necessary to fully qualify the hostname with the DNS suffix, e.g. 0030d3177321.country.company.com. 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conventions. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 210: Setup (Dhcp)

  0030d3177321. On the Local Controller the MAC address can be found under Details in the LAN section. Figure 29 LAN Setting on Instant Pilot InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 211
  Lab Advisor, Firmware Update Tool) and use MAC address as host name, e.g. 0030d3177321. The LC system should become visible in the control software (see Note in section “General Information (DHCP)” on page 209). InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 212: Manual Configuration

  Telnet session. 1 Open the system (DOS) prompt window by clicking on Windows START button and select “Run…”. Type “cmd” and press OK. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 213
  Handheld Controller, or the default IP address (see “Configuration Switches” on page 206). When the connection was established successfully, the module responds with the following: Figure 32 A connection to the module is made InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 214
  Then press [Enter], where parameter refers to the configuration parameter you are defining, and value refers to the definitions you are assigning to that parameter. Each parameter entry is followed by a carriage return. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 215
  Initialization mode is Using Stored active TCP/IP settings stored TCP/IP settings in non-volatile memory connected to PC with controller software (e.g. Agilent ChemStation), here not connected Figure 35 Telnet — Change IP settings InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 216
  If the Initialization Mode Switch is changed now to “Using Stored” mode, the instrument N O T E will take the stored settings when the module is re-booted. In the example above it would be 192.168.254.12. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 217: Appendix

  InfinityLab LC Series 1260 Infinity II Binary Pump User Manual Appendix General Safety Information General Safety Information Safety Standards General Before Applying Power Ground the Instrument Do Not Operate in an Explosive Atmosphere Do Not Remove the Instrument Cover Do Not Modify the Instrument…

• Page 218: General Safety Information

  Do not use this product in any manner not specified by the manufacturer. The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 219: Before Applying Power

  Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. ➔…

• Page 220: Do Not Operate In An Explosive Atmosphere

  Personal injury (for example electrical shock, intoxication) ➔ Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 221: Solvents

  For details, see the usage guideline for the solvent cabinet. A printed copy of the guideline N O T E has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 222: Safety Symbols

  European Community directives. The European Declaration of Conformity is available at: http://regulations.corporate.agilent.com/DoC/search.htm Manufacturing date. Power symbol indicates On/Off. The apparatus is not completely disconnected from the mains supply when the power switch is in the Off position InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 223
  ➔ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 224: Waste Electrical And Electronic Equipment (Wee) Directive

  Do not dispose of in domestic household waste N O T E To return unwanted products, contact your local Agilent office, or see http://www.agilent.com for more information. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 225: Radio Interference

  If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises. InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 226: Sound Emission

  This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 227: Agilent Technologies On The Internet

  Appendix Agilent Technologies on the Internet Agilent Technologies on the Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…

• Page 228
  CAN partner DHCP motor-drive power general information overview overpressure 102, 106, 104, setup RS-232 pressure above upper limit dimensions InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 229
  TCP/IP parameter configuration minimum using default motor-drive power frequency range using stored InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 230
  InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 231
  InfinityLab LC Series 1260 Infinity II Binary Pump User Manual…
• Page 232
  In This Book This manual contains technical reference information about the Agilent 1260 Infinity II Binary Pump G7112B. The manual describes the following: • introduction, • site requirements and specifications, • using the binary pump, • optimizing performance, • troubleshooting and diagnostics, •…

• Page 1
  Agilent InfinityLab LC Series 1260 Infinity II Preparative Autosampler User Manual Agilent Technologies…
• Page 2
  (June 1987) or any equivalent agency regu- lation or contract clause. Use, duplication or disclosure of Software is subject to Agi- lent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 3
  Overview about the troubleshooting and diagnostic features. 8 Error Information This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. Agilent 1260 Infinity II Preparative Autosampler User Manual…
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  This chapter describes the detector in more detail on hardware and electronics. 13 LAN Configuration This chapter provides information on connecting the module to the Agilent ChemStation PC. 14 Appendix This chapter provides addition information on safety, legal and web. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 5: Table Of Contents

  Transporting the Sampler with a Sample Cooler Installed Agilent Local Control Modules 4 Installation of the Optional Sample Cooler Injector Step Commands Damaged Packaging Install the Sample Cooler Using the Sample Cooler in an Infinity II Sampler Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 6
  Step Commands Tests Troubleshooting Troubleshooting Guide for the Sample Transport Assembly Agilent Lab Advisor Software 8 Error Information What are Error Messages General Error Messages Sampler Error Messages Sample Cooler Error Messages Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 7
  Standard Parts Drawer Assembly External Tray Analytical-Head Assembly (900 µL) Standard Sampler Accessory Kit Multi-Draw Kit Additional Part List 11 Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 8
  Dynamic Host Configuration Protocol (DHCP) Manual Configuration PC and Agilent ChemStation Setup 14 Appendix General Safety Information Waste Electrical and Electronic Equipment Directive Refrigerant Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 9: Introduction

  Agilent 1260 Infinity II Preparative Autosampler User Manual Introduction Product Description Features Overview of the Module Operating Principle Sampling Sequence Needle Parkstation Hydraulic Box Transport Assembly Leak and Waste Handling Leak Sensor Waste Concept This chapter gives an introduction to the module and instrument overview.

• Page 10: Product Description

  Introduction Product Description Product Description The 1260 Infinity II Preparative Autosampler is designed for laboratories that need automated sample injection to increase daily throughput of purified sample. This autosampler is easy to use, reliable, and capable of injecting sample volumes from vials in the microliter to milliliter range.

• Page 11: Features

  • Support of flow rates up to 100 mL/min at 400 bar Laboratory efficiency • Maximum capacity of 132 sample vials within the same foot print • Flexible and convenient sample handling with different types of sample containers Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 12: Overview Of The Module

  Overview of the Module Overview of the Module The Agilent 1260 Infinity II Preparative Autosampler is designed for use with other modules of the Agilent 1200 Infinity Series, 1200 Series, and 1100 Series LC, or with other LC systems if adequate remote control inputs and outputs are available.

• Page 13: Operating Principle

  If this feature is disabled, the gripper arm positions the sample vial directly below the needle (Step 4) and lowers the needle into the vial. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 14
  This ensures that all parts in contact with sample are flushed during the run, thus minimizing carry-over. Figure 2 Mainpass Position — standard position during runs and when the sampler is idle Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 15
  15). Solvent from the pump enters the valve unit at port 1, and flows directly to the column through port 6. Figure 3 Valve in bypass — needle in vial, metering device aspirates sample volume Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 16
  In the next step, the needle is washed (see Figure 4 page 16). Figure 4 Outer face of needle getting washed in wash port Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 17
  (see Figure 5 on page 17). Figure 5 Valve switches to main pass — sample is transferred towards the LC column Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 18: Needle Parkstation

  The upper and lower needle positions are detected by reflection sensors on the needle station board, while the needle-in-vial position is determined by counting the motor steps from the upper needle-sensor position. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 19: Hydraulic Box

  The backward movement of the piston (driven by the spring) draws sample from the vial. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 20
  Two microswitches monitor switching of the valve (bypass and mainpass end positions). No valve adjustments are required after replacing internal components. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 21: Transport Assembly

  These positions are used by the processor to calculate the actual motor position. An additional six reflection sensors for tray recognition are mounted on the flex board at the front of the assembly. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 22: Leak And Waste Handling

  PVDF (polyvinylidene fluoride), is incompatible with DMF. ➔ Do not use DMF. The leak sensor in the sampler is hidden under the ICC Column Heater or Column Shelf N O T E respectively. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 23: Waste Concept

  1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don’t immerse in the liquid. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 24
  Introduction Leak and Waste Handling Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 25: Site Requirements And Specifications

  Agilent 1260 Infinity II Preparative Autosampler User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications Specifications of the Sample Cooler This chapter provides information on environmental requirements, physical and performance specifications. Agilent Technologies…

• Page 26: Site Requirements

  ➔ Make sure the power connector of the instrument can be easily reached and unplugged. ➔ Provide sufficient space behind the power socket of the instrument to unplug the cable. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 27
  Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Power cords WA R N I N G Solvents may damage electrical cables.
• Page 28
  ➔ If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 29: Physical Specifications

  Auto-ignition temperature ≥200 °C If a sample cooler is included the upper value for humidity can be reduced. Please check your lab conditions to stay beyond dew point values for non–condensing operation. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 30: Performance Specifications

  Site Requirements and Specifications Performance Specifications Performance Specifications Table 2 Performance Specifications 1260 Infinity II Preparative Autosampler (G7157A) Type Specification Comment Settable injection 0.1 – 900 µL, up to 3600 µL with seat extension range 1 µL: <5 % Injection precision Measured caffeine 5 µL: <2 %…

• Page 31
  Site Requirements and Specifications Performance Specifications Table 2 Performance Specifications 1260 Infinity II Preparative Autosampler (G7157A) Type Specification Comment Instrument Control LC & CE Drivers A.02.17 or above For details about supported software versions Instrument Control Framework (ICF) A.02.04 or…

• Page 32: Specifications Of The Sample Cooler

  Up to 4600 m (15091 ft) Safety standards: Installation category II, For indoor use only. IEC, EN, CSA, UL Pollution degree 2 ISM Classification ISM Group 1 Class B According to CISPR 11 Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 33
  4 °C to 5 °C below ambient Temperature settable from 4 – 40 °C in 1 ° increments Temperature accuracy (<25 °C, <50 % r.H.) 2 °C to 6 °C at a setpoint of 4 °C Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 34
  Site Requirements and Specifications Specifications of the Sample Cooler Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 35: Using The Module

  Agilent 1260 Infinity II Preparative Autosampler User Manual Using the Module Magnets Turn on/off Status Indicators Exchange Drawers Install the External Tray Sample Trays Choice of Vials and Caps Transporting the Sampler with a Sample Cooler Installed Agilent Local Control Modules This chapter provides information on how to use the module.

• Page 36: Magnets

  Using the Module Magnets Magnets This stack exemplarily shows the magnets’ positions in the modules. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 37: Turn On/Off

  Using the Module Turn on/off Turn on/off Power switch: On Turn instrument On/Off with the control software. Power switch: Off Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 38
  Using the Module Turn on/off Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 39: Status Indicators

  5. Resident mode (blinking) — for example during update of main firmware. 6. Bootloader mode (fast blinking). Try to re-boot the module or try a cold-start. Then try a firmware update. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 40: Exchange Drawers

  Install classic drawer 1-50 to the left, classic drawer 51-100 to the right side. Open the front door. Pull the drawer out. Lift the front of the drawer. Lift the drawer out. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 41
  Using the Module Exchange Drawers Remove the drawer. Insert the back of the drawer. Align the drawer. Push in the drawer. Close the doors. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 42: Install The External Tray

  For best temperature performance, and if the external tray is not in use, it is best to cover the opening for the external tray with the original parts. Lift the foam with a flathead screwdriver. Remove the foam. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 43
  Using the Module Install the External Tray Push out the plastic cover. Insert the external tray. Vent for the external tray is open. Install the disposal tube. Close the door. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 44: Sample Trays

  Drawers can be installed in any combination enabling both 2 mL- and 6 mL-vials to be used simultaneously. The only exception is the usage of the classical drawer option (100 x 2 mL). This option can’t combine with the other drawers. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 45
  No. 206) Figure 9 Numbering of drawer position (left-hand Drawer for 66 x 2 mL Vials) Figure 10 Numbering of drawer position (right-hand Drawer for 66 x 2 mL Vials) Agilent 1260 Infinity II Preparative Autosampler User Manual…
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  Numbering of drawer position (left-hand Drawer for 18 x 6 mL Vials) Figure 12 Numbering of drawer position (right-hand Drawer for 18 x 6 mL Vials) Figure 13 Numbering of drawer position (Drawer for 50 x 2 mL Vials Classic) Agilent 1260 Infinity II Preparative Autosampler User Manual…
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  Using the Module Sample Trays Figure 14 Numbering of tray position (External tray) Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 48: Choice Of Vials And Caps

  (silanized) 5182-0567 Crimp Top Vial, 1 mL, polypropylene, wide opening, 100/Pack 5183-4496 Crimp Top Vial, 1 mL, polypropylene, wide opening, 100/Pack (silanized) 9301-0978 crimp top vial, 0.3 mL, polypropylene, wide opening, 1000/pack Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 49
  Screw Cap Vial, 2 mL, amber glass, write-on spot, 100/pk 5183-2069 Screw Top Vial, 2 mL, amber glass, write-on spot, 1000/Pack 5183-2072 Screw Top Vial, 2 mL, amber glass, write-on spot, 100/Pack (silanized) Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 50
  Snap Cap, clear polypropylene, septum (clear PTFE/red rubber), 100/Pack 5182-3458 Snap Cap, blue polypropylene, septum (clear PTFE/red rubber), 100/Pack 5182-3457 Snap Cap, green polypropylene, septum (clear PTFE/red rubber), 100/Pack 5182-3459 Snap Cap, red polypropylene, septum (clear PTFE/red rubber), 100/Pack Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 51
  Screw Cap, red polypropylene, septum (clear PTFE/red rubber), 100/Pack 5182-0720 Screw Cap, blue polypropylene, septum (clear PTFE/silicone), 100/Pack 5182-0721 Screw Cap, green polypropylene, septum (clear PTFE/silicone), 100/Pack 5182-0722 Screw Cap, red polypropylene, septum (clear PTFE/silicone), 100/Pack Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 52: Transporting The Sampler With A Sample Cooler Installed

  Ensure that you can cope with the weight of your load. Transporting the sampler with a sample cooler installed is only allowed for short N O T E distances. For longer distances, you must separate the units and send them independently. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 53
  Advisor online help for more information. Also ensure there is no vial in the gripper arm. • Install the transport protection. • Remove all vials from the drawers. • The condensed water inside of the sample cooler is removed. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 54: Agilent Local Control Modules

  • fast and direct control in front of the instrument • a clear overview of the system status • control functionalities • access to method parameters and sequences • a logbook showing events from the modules • diagnose tests Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 55
  • GLP – System logbook and module log-books record errors, unusual events and maintenance activities for GLP traceability. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 56
  Using the Module Agilent Local Control Modules Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 57: Installation Of The Optional Sample Cooler

  Agilent 1260 Infinity II Preparative Autosampler User Manual Installation of the Optional Sample Cooler Injector Step Commands Damaged Packaging Install the Sample Cooler Using the Sample Cooler in an Infinity II Sampler Control Interface Control Temperature Mode Online Signal Monitor This chapter describes the installation of the optional sample cooler.

• Page 58: Injector Step Commands

  Needle into Seat Lowers the needle arm into the seat. Command also returns the vial to the tray position. Valve Mainpass Switches the injection valve to the mainpass position. Reset Resets the injector. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 59: Damaged Packaging

  Damaged Packaging Damaged Packaging If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. «Defective on arrival» problems C AU T I O N If there are signs of damage, please do not attempt to install the module.

• Page 60: Install The Sample Cooler

  A suitable container must be provided and emptied regularly in order to avoid overflow. For best cooling performance of the sampler all drawers must be installed in the module. N O T E Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 61
  Ensure that the power switch on the front of the module Ensure that the power cable is removed from the is OFF (switch stands out). instrument. Open the 4 screws on the rear of the module. Remove the sample cooler mainframe cover. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 62
  To avoid damages of the electronics of the module make sure the power cords are unplugged before disconnecting or reconnecting the sampler to the sample cooler cables. Connect power cable and signal/data cable. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 63
  Fit in the sample cooler perfectly. Slide in the whole unit. Use a bubble level to check the leveling of the sampler. N O T E The sample cooler should be operated in a proper horizontal position. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 64
  Wait at least 30 min before switching on the compressor of the sample cooler. ➔ This allows the refrigerant and system lubrication to reach equilibrium. Connect the power cable to the power connector at the rear of the module. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 65: Using The Sample Cooler In An Infinity Ii Sampler

  Drawer Configuration EMF Status ALS configuration is displayed when moving with the mouse cursor over the syringe. The information provides ALS related information like • Part number • ALS setup and other details. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 66: Control Interface

  Installation of the Optional Sample Cooler Using the Sample Cooler in an Infinity II Sampler Control Interface A right-click into the active area will open a menu to show the Control Interface. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 67: Control

  Select On to switch on the cooler. Specify the required temperature in the adjacent field. Note that the specified temperature must be at least 5 °C below ambient for proper temperature control. Select Off to switch off the cooler. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 68: Temperature Mode

  This mode may be used for degradation experiments (this solution is not officially recommended by Agilent) or as a workaround for reporting. Reporting of control parameters will become available with the CDS and ICF. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 69: Online Signal Monitor

  The online signal monitor for the chiller is also available and allows you to monitor the WPS1A temperature signals. The Online Plot Window should look like this: If the change button is pressed you can select the Chiller signal WPS1A in the online plot. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 70
  This will disappear after the unit comes out of the heating cycle and goes back into normal operation. Shut down of the sample cooler at low temperatures will build up some condensate inside the autosampler. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 71
  Adjusting the set points from a colder to a warmer set point will result in some N O T E condensation. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 72
  Installation of the Optional Sample Cooler Using the Sample Cooler in an Infinity II Sampler Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 73: Preparing The Module

  Agilent 1260 Infinity II Preparative Autosampler User Manual Preparing the Module Leak and Waste Handling Preparing the Module Solvent Information Capillary Color Coding Guide Swage Fittings Flow Connections to the sampler Setting up the Sampler Control Settings Method Parameter Settings…

• Page 74: Leak And Waste Handling

  N O T E has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet. For details on correct installation, see separate installation documentation. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 75: Preparing The Module

  To clean the system when Bidistilled water Best solvent to re-dissolve buffer using buffers crystals After a solvent change Bidistilled water Best solvent to re-dissolve buffer crystals Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 76: Solvent Information

  HPLC analysis over several decades. These materials exhibit excellent robustness under typical HPLC conditions. For any special condition, please consult the material information section or contact Agilent. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 77
  PEEK capillaries keeps the flow path free of steel and ensures pressure stability to at least 600 bar. If in doubt, consult the available literature about the chemical compatibility of PEEK. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 78
  2.3. It can also corrode in following solvents: • Solutions of alkali halides, their respective acids (for example, lithium iodide, potassium chloride, and so on) and aqueous solutions of halogens. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 79
  . Titanium is subject to corrosion in anhydrous chlorides like FeCl methanol, which can be avoided by adding a small amount of water (about 3 %). Slight corrosion is possible with ammonia > 10 %. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 80
  (perfluoroalkoxy) and FEP (fluorinated ethylene propylene) are inert to almost all common acids, bases, and solvents. FFKM is perfluorinated rubber, which is also resistant to most chemicals. As an elastomer, it may swell in some organic solvents like halogenated hydrocarbons. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 81
  Sapphire, Ruby and Al -based ceramics Sapphire, ruby and ceramics based on aluminum oxide Al are inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 82: Capillary Color Coding Guide

  Preparing the Module Capillary Color Coding Guide Capillary Color Coding Guide Figure 15 Syntax for capillary description Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 83: Swage Fittings

  N O T E Don’t overtighten. Overtightening will shorten the lifetime of the fitting. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 84
  If changing from a column or an injection valve to another, the fitting may leak or decrease the quality of the separation by contributing to band broadening. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 85: Flow Connections To The Sampler

  4 Route the drainage of the washport outlet to the waste container. 5 Prime the wash tubing. 6 Install the capillary from the pump outlet into the port 1 of the injection valve. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 86
  7 Install the capillary from port 6 of the injection valve to the ICC or MCT The correct plumbing is shown in the figure below and is attached to the module. 8 Prime and purge the complete sampler. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 87: Setting Up The Sampler

  • Needle Wash: allows you to wash the needle without modifying your current method. The needle wash specified in this dialog box is carried out immediately. • Modify • Flow Path • External Tray • Temperature Mode Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 88
  EMF Status shows Run / Ready / Error state and “Not Ready text” or “Error text” • Offline (gray) • Ok No Maintenance required (green) • EMF warning. Maintenance might be required (yellow) • EMF warning. Maintenance required (red) Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 89
  Preparing the Module Setting up the Sampler Figure 17 ChemStation Method and Run Control Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 90: Control Settings

  For additional help and support, highlight the desired area and press the F1 key. A help N O T E screen will open with additional information and documentation about the topic. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 91: Method Parameter Settings

  Preparing the Module Setting up the Sampler Method Parameter Settings The method parameter settings are available via right click on the active area of the grafical user interface. Figure 19 Method parameter settings Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 92
  Posttime to delay the start of the next analysis. A Posttime period can be used to allow the column to equilibrate after changes in solvent composition (for example after gradient elution). Limits: 0.01 through 99999 min or Off. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 93
  (in mm) away from its standard position. The Offset function is useful when analyzing very small sample volumes, or when only a specific part of the sample is required, for example, the top layer. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 94
  For additional help and support, highlight the desired area in the user interface and press N O T E the F1 key. A help screen will open with additional information and documentation about the topic. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 95: Injector Programm

  The following functions are available : • Draw • Eject • Mix • Inject • Move • Wait • Valve • Needle • Wash • Remote • Wait For • Repeat . End Repeat • Comment Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 96: Module Configuration View

  N O T E screen will open with additional information and documentation about the topic. The vialsampler configuration is done in the module dashboard context menu, not in the N O T E instrument configuration. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 97: Optimizing Performance

  Agilent 1260 Infinity II Preparative Autosampler User Manual Optimizing Performance Optimization for Lowest Carry-over Using the Automated Needle Wash (Wash Port) Using an Injector Program General Recommendation to Lowest Carry-Over Fast Injection Cycle and Low Delay Volume Overlapped Injection Mode…

• Page 98: Optimization For Lowest Carry-Over

  It has a volume of ca. 0.5 mL and the peristaltic pump delivers ca. 5 mL/min, which means the wash port volume is completely refilled with fresh solvent in ca. 6 s. If the wash port is selected, the user can set the length Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 99
  Line 1 moves the needle to vial P2-A1 or the flushport. Line 2 draws 5 μL from the current sample vial P1-A1. Line 3 injects the sample (valve switches to main pass). Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 100: Using An Injector Program

  Wait (equilibration time — see text above) Valve bypass Wait 0.2 min Valve mainpass Valve bypass Valve mainpass Overlapped injection together with additional injection valve switching is not possible. N O T E Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 101: General Recommendation To Lowest Carry-Over

  • Clean the needle seat with a tissue and remove all liquid. Again be careful not to risk an injury by the uncovered needle. • Reinstall the safety cover of the sampler. • In LabAdvisor, go to Maintenance > Change Needle and finish the procedure. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 102: Fast Injection Cycle And Low Delay Volume

  100 μl instead of 1 μl, increase the injection time by approximately 8 sec. In this case and if the viscosity of the sample allows it, the draw and eject speed of the injection system has to be increased. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 103: General Recommendations For Fast Injection Cycle Times

  • Increase of draw and eject speed for large injection volumes • Add at last run a blank, if overlapped injection is used To reduce the injection time, the detector balance has to be set to OFF. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 104: Precise Injection Volume

  A DRAW statement in an injector program also uses the draw speed setting which is configured for the autosampler. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 105
  An EJECT statement in an injector program also uses the eject speed setting which is configured for the autosampler. A faster eject speed shortens the time required to run the injector program. When using viscous samples, a high eject speed should be avoided. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 106: Choice Of Rotor Seal

  It is suitable for application using mobile phases within a pH between 1 and 14. Strong oxidizing acids such as concentrated nitric and sulfuric acids are not compatible N O T E with PEEK. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 107: Troubleshooting And Diagnostics

  Agilent 1260 Infinity II Preparative Autosampler User Manual Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Maintenance Functions User Interface Change Needle Change Metering Seal or Piston Park Arm Change Gripper Automatic Referencing Sampler Gripper Verification Test…

• Page 108: Overview Of The Module’s Indicators And Test Functions

  Tray alignment is required after repair of internal components. The procedure aligns the gripper arm correctly to ensure the positioning of the gripper arm is correct for all vials (see “Automatic Referencing” on page 117). Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 109
  The step functions provide the possibility to execute each step of the sampling sequence individually. The step functions are used primarily for troubleshooting, and for verification of correct autosampler operation after repair (see “Step Commands” on page 119). Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 110: Maintenance Functions

  Change Gripper: The change gripper function moves the gripper to the front of the autosampler enabling easy access to the gripper release mechanism. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 111: Change Needle

  The needle arm moves in the desired position and guide the user to the next procedure step. Read carefully the desciption in the LabAdvisor user interface. N O T E Move Down The needle arm moves slowly down. Move Up The needle arm slowly moves up. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 112
  5 Next positions the needle to the top level. Flap the safety wash port back in its origin position. 6 Reinstall the safety cover and complete the procedure. 7 Select Next to move the needle into the needle seat. 8 Select Back to complete the procedure. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 113: Change Metering Seal Or Piston

  3 Select Next to move the piston back to the home position. Read carefully the description in the LabAdvisor user interface. N O T E 4 Select Back to finish the procedure. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 114: Park Arm

  Control display. The commands for the control software are: Park Arm moves the gripper arm to the park position. Home moves the gripper arm out of the park position to the home position. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 115
  Gripper function to remove the vial. 1 Select Park Arm. 2 When the arm is in the park position, install Transport-Protection (G7129-40050) . Then the autosampler is ready for shipment, and can be switched OFF. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 116: Change Gripper

  2 Exchange the gripper arm (see “Exchange the Gripper Arm” on page 177). 3 Select Next to move the gripper arm to the home position. 4 Select Back to finish the gripper procedure. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 117: Automatic Referencing

  Mount the calibration fixture. Position P1-C6 and P2-F6. Please check help for further explanation. N O T E Next Mount a 2 mL vial. Next Perform autoreferencing. Next Remove calibration fixture. Back Completes the procedure. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 118: Sampler Gripper Verification Test

  Automatic Referencing procedure should be done. Allows you to select a vial position. You can select from positions 1, 11, 39, 56, 66, 101, 111, 139, 156 and 166. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 119: Step Commands

  Needle into Seat Lowers the needle arm into the seat. Command also returns the vial to the tray position. Valve Mainpass Switches the injection valve to the mainpass position. Reset Resets the injector. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 120: Tests

  0.5 °Cover a 10 s period) the cooler is turned on and measurement is started. For the test to succeed, three temperature checkpoints must be reached within a specified time. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 121: Troubleshooting

  You can use the injector steps to do the injection sequence, while observing how the instrument responds. “Automatic Referencing” on page 117 summarizes the injector steps, and lists the associated error messages and probable causes of step failures. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 122: Troubleshooting Guide For The Sample Transport Assembly

  • motor overtemp (0 or 2 or 3) • movement failed (0 or 2 or 3) • missing vial Motor 0=X; 1=Z; 2=Theta; 3=Gripper. N O T E Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 123: Intermittent Lock-Ups With Or Without Vial In The Gripper Fingers

  (G7129-65800)) or the fusion board ( Fusion Board (G4200-65880)). 7 For both steps (step 5 and 6), you have to do an automatic referencing again to store the correct values in the instrument firmware again. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 124: Poor Alignment

  (G7129-65800)) or the fusion board ( Fusion Board (G4200-65880)). 5 For all three steps (step 2, 3, and 4) you have to do an automatic referencing again to store the correct values in the instrument firmware again. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 125: Agilent Lab Advisor Software

  The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual. For details, refer to the Agilent Lab Advisor software help files. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 126
  Troubleshooting and Diagnostics Agilent Lab Advisor Software Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 127: Error Information

  Agilent 1260 Infinity II Preparative Autosampler User Manual Error Information What are Error Messages General Error Messages Timeout Shutdown Remote Timeout Lost CAN Partner Leak Sensor Leak Sensor Open Leak Sensor Short Compensation Sensor Open Compensation Sensor Short Fan Failed…

• Page 128
  Cooler temperature below or above target temperatur. Cooler Overpressure Failure Cooler Condensate Error This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 129: What Are Error Messages

  In all cases, error propagation is done via the CAN bus or via an APG/ERI remote cable (see documentation for the APG/ERI interface). Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 130: General Error Messages

  Check the vacuum degasser for an error The degasser failed to generate sufficient condition. Refer to the Service Manual for the vacuum for solvent degassing. degasser or the pump that has the degasser built-in. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 131: Remote Timeout

  Exchange the CAN cable. Defective CAN cable. Switch off the system. Restart the system, and Defective main board in another module. determine which module or modules are not recognized by the system. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 132: Leak Sensor

  Exchange the peristaltic pump. Leaking peristaltic pump The leak sensor in the sampler is hidden under the ICC Column Heater or Column Shelf N O T E respectively. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 133: Leak Sensor Open

  Switch board. Exchange the leak sensor. Defective leak sensor. Exchange the leak sensor. Leak sensor incorrectly routed, being pinched by a metal component. Exchange the power switch assembly. Power switch assembly defective Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 134: Leak Sensor Short

  Check the ribbon cable between the power Loose connection between the power switch board and the main board. switch board and the main board Exchange the power switch assembly. Defective power switch assembly Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 135: Compensation Sensor Short

  Probable cause Suggested actions Ensure the fan is connected correctly. Fan cable disconnected. Exchange fan. Defective fan. Replace the sample cooler. Defective sample cooler fan Exchange the main board. Defective main board. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 136: Sampler Error Messages

  If the valve fails to reach the mainpass position, or if the microswitch does not close, the error message is generated. Probable cause Suggested actions Exchange the injection valve. Defective injection valve. Exchange the main board. Defective main board. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 137: Needle Movement Failed

  Align gripper. Incorrect gripper alignment. Exchange the gripper assembly. Defective gripper assembly (defective gripper fingers or belt). Exchange the transport assembly. Defective transport assembly flex board. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 138: Initialization Failed

  Exchange and reset the needle station board. Defective needle station board. Exchange the transport assembly. Defective transport assembly flex board. Exchange the hydraulic unit motor. Defective hydraulic unit motor. Exchange the main board. Defective main board. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 139: Metering Home Failed

  Exchange the metering piston and seal. Broken piston. Exchange the metering-drive motor. Defective metering-drive motor. Exchange the main board. Defective main board. • Check belt tension. Loose belt. • Exchange the belt Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 140: Motor Temperature

  Switch OFF the autosampler at the power Motor belt tension too high. switch. Wait at least 10 min before switching on again. Exchange the transport assembly. Defective motor. Exchange the transport assembly. Defective transport assembly flex board. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 141: Initialization With Vial

  Probable cause Suggested actions Remove the vial using the Release Vial Vial still in gripper. function in the user interface. Reinitialize the autosampler. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 142: Missing Wash Vial

  Tray recognition defective (dirty sample tray are clean (located at the rear of the sample or defective transport assembly flex board). tray). If this does not solve the problem, replace the transport assembly. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 143: Valve Switch Not Found

  (mainpass or bypass), or if the sensor fails to recognize the valve position, the error message is generated. Probable cause Suggested actions Exchange the rotor seal/stator head. Defective rotor seal/stator head. Exchange the injection valve. Defective injection valve. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 144: Sample Cooler Error Messages

  • Check the power cable from the ALS to the Blown fuses, defective cable sample cooler. • Check if a fuse is blown (LED status on the specific mainboard). Replace the sample cooler. Defective PCB boards Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 145
  Probable cause Suggested actions Switch off the instrument and wait for 15 min. Overpressure in the chiller Restart the instrument and check the signals in Lab Advisor. Replace the sample cooler. Defective compressor Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 146
  • Switch the sample cooler off/on and check Defective condensate sensor if after 8 min the error shows up again. • Replace the condensate sensor. Replace the sample cooler. Defective leak sensor Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 147: Maintenance

  Agilent 1260 Infinity II Preparative Autosampler User Manual Maintenance Introduction to Maintenance Warnings and Cautions Overview of Maintenance Cleaning the Module Remove and Install Doors Exchange the Needle Assembly Exchange the Needle Seat Assembly Exchange the Sample Loop Assembly Exchange the Rotor Seal…

• Page 148: Introduction To Maintenance

  Status indicator Needle assembly Drawer Column shelf Leak drain Power switch There are no serviceable parts inside. N O T E Do not open the module. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 149: Warnings And Cautions

  Sharp metal edges WA R N I N G Sharp-edged parts of the equipment may cause injuries. ➔ To prevent personal injury, be careful when getting in contact with sharp metal areas. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 150
  Avoid back strain or injury by following all precautions for lifting heavy objects. ➔ Ensure that the load is as close to your body as possible. ➔ Ensure that you can cope with the weight of your load. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 151: Overview Of Maintenance

  Table 11 Overview of maintenance Procedure Typical interval (minimum) Notes Change needle/needle seat 30000 needle into seat movements Change peristaltic pump 3000 min on time cartridge Change rotor seal 30000 injections Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 152: Cleaning The Module

  WA R N I N G hazard and damage the module ➔ Do not use an excessively damp cloth during cleaning. ➔ Drain all solvent lines before opening any connections in the flow path. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 153: Remove And Install Doors

  Press the release buttons and pull the front door out. For the Installation of the front door, insert the hinges into their guides and move the door in until the release buttons click into their final position. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 154: Exchange The Needle Assembly

  Wear safety goggles and safety gloves when removing the needle assembly. It is recommended to always exchange the needle assembly and the needle seat at the N O T E same time to prevent premature leakage. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 155
  Unscrew the safety cover screw. WA R N I N G Sharp needle Uncovered needles may cause injuries ➔ Do not touch the tip of the needle. Remove the safety cover. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 156
  Turn or flap the wash port to the left. The needle will move slowly downwards and will stop approximately 2 mm above the seat. Disconnect the sample loop from the needle. Loosen the fixing screw. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 157
  N O T E Do not overtighten the fitting! N O T E Incorrect positioning and installation of the needle/loop connection can result in damaging and breaking the sample loop. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 158
  Install the safety cover (1) and fix the screw (2). Select Next to move the needle into the needle seat. Select Back to finish the procedure. Close the doors. Perform a pressure test. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 159: Exchange The Needle Seat Assembly

  In the Agilent Lab Advisor software select Service & Open the front door. Diagnostics in the system screen (Tools) Maintenance Positions > Change Needle/Loop, click Start and wait until the needle assembly is in maintenance position. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 160
  Do not touch the tip of the needle. Turn or flap the wash port to the left. The needle will move slowly downwards and will stop approximately 2 mm above the seat. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 161
  Disconnect the seat-capillary fitting from the injection valve (port 5). Use a small flat-head screwdriver to ease out the needle Insert the new needle-seat assembly. Press the seat seat. firmly into position. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 162
  N O T E Do not overtighten the fitting! N O T E Incorrect positioning and installation of the needle/loop connection can result in damaging and breaking the sample loop. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 163
  Install the safety cover (1) and fix the screw (2). Select Next to move the needle into the needle seat. Close the doors. Select Back to finish the procedure. Perform a pressure test. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 164: Exchange The Sample Loop Assembly

  Change loop function. In the Agilent Lab Advisor software select Service & Diagnostics in the system screen Maintenance Positions > Change loop, click Start and wait until the needle assembly is in maintenance position. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 165
  Do not touch the tip of the needle. Remove the safety cover. Check if the needle is in maintenance position. Disconnect the loop capillary from the metering device and from the needle. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 166
  Install the safety cover (1) and fix the screw (2). Click Next until the needle assembly is moved to the Close the doors. needle seat. Select Back to finish the procedure. Perform a pressure test. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 167: Exchange The Rotor Seal

  The stator face is held in place by the stator head. When you remove the stator head, the stator face can fall out of the valve. ➔ Carefully handle the valve to prevent damage to the stator face. ➔ Carefully handle the stator face during sonication. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 168
  Remove all capillaries from the injection valve with a 1/4 inch wrench. Loosen each fixing bolt two turns at a time. Remove the Remove the stator head and stator ring. bolts from the head. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 169
  N O T E Make sure that the rotor sealing surface with its engraved flow passages is facing out. The pattern is asymmetrical to prevent improper placement. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 170
  Do not over-tighten the screws. The screws hold the assembly together and do not affect the sealing force. The sealing force is automatically set as the screws close the stator head against the valve body. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 171
  Reconnect the capillaries and tubes to the valve ports. Slide the waste tube into the waste holder in the leak tray. The correct plumbing is shown below and on the safety cover of the module. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 172
  Maintenance Exchange the Rotor Seal Close the doors. Perform a pressure test. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 173: Exchange The Metering Seal And Piston

  In the Tools section of the Agilent Lab Advisor software Open the front door. select Service & Diagnostics > Maintenance Positions > Change Metering Device, click Start and wait until the metering device is in maintenance position. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 174
  Pull the metering head assembly away from the sampler. Remove the two fixing bolts from the base of the metering head assembly. Disassemble the metering head assembly. Use a seal insert tool to carefully remove the seal. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 175
  Ensure the large hole in the metering head is facing stator head is secure. The housing must flush with the downwards. Tighten the bolts alternately two turns at a metering head. time until the metering device is secure. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 176
  Service & Diagnostics > Maintenance Positions > Change Metering Device: click Next and wait until the metering device is in Home position. Select Back to finish the procedure. Close the doors. Perform a pressure test. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 177: Exchange The Gripper Arm

  Identify the slit below the gripper motor and the gripper Rotate the arm approximately 2.5 cm (1 inch) to the left arm release button. and insert the straightened paper clip into the slit. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 178
  Close the doors. In the Agilent Lab Advisor software select Change gripper > Next, click Next and wait until the gripper is in the default position. Select Back to finish the procedure. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 179: Replace The Peristaltic Pump Cartridge

  The peristaltic pump cartridge is a replaceable unit. The tubing inside the pump is not N O T E replaceable. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 180
  Push the new cartridge onto the motor shaft until the Connect the wash port tubing to the upper tubing of the clips click into place. new cartridge (use sand paper to get a good grip on the tubing). Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 181
  Connect the inlet filter of the solvent bottle. Use the Close the doors. syringe to draw enough solvent for completely filling of the peristaltic pump tubing before continuing to prime the peristaltic pump. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 182: Exchange The Wash Port Assembly

  Diagnostics in the system screen (Tools) Maintenance Positions > Change Needle/Loop, click Start and wait until the needle assembly is in maintenance position. Check if the needle is lifted up. Unscrew the safety cover screw. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 183
  In Lab Advisor use Next to move the needle in the down Disconnect the sample loop from the needle. position. The needle will move slowly downwards and will stop approximately 2 mm above the seat. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 184
  Disconnect the wash tubing from the peristaltic pump. Release the spring loaded pin. Lift the flapper out of the bracket. Be careful not to Reconnect the wash tubing fitting to the new wash port squeeze the wash tubing. assembly. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 185
  (2). N O T E Do not overtighten the fitting! N O T E Incorrect positioning and installation of the needle/loop connection can result in damaging and breaking the sample loop. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 186
  Select Next to move the needle into the needle seat. Select Back to finish the procedure. Close the doors. Purge the wash port. Check if solvent will be delivered from the peristaltic pump. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 187: Replace The Module Firmware

  Agilent web. http://www.agilent.com/en-us/firmwareDownload?whid=69761 2 For loading the firmware into the module follow the instructions in the documentation. Module Specific Information There is no specific information for this module. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 188
  Maintenance Replace the Module Firmware Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 189: Parts And Materials For Maintenance

  Agilent 1260 Infinity II Preparative Autosampler User Manual Parts and Materials for Maintenance Main Assemblies Standard Parts Drawer Assembly External Tray Analytical-Head Assembly (900 µL) Standard Sampler Accessory Kit Multi-Draw Kit Additional Part List This chapter provides information on parts for maintenance.

• Page 190: Main Assemblies

  Drawer for 50 x 2 mL Vials Classic Right 5067-4277 2pos/6port Injection Valve, prep, 600 bar G1313-60010 Gripper assembly G7129-60083 Analytical Head Assembly 900 µL 5065-4445 Peristaltic pump with Pharmed tubing Figure 21 Main assemblies Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 191: Standard Parts

  Finger caps (x3) G7157-60515 Loop for Prep-Autosampler G7157-87101 Seat Assy High Flow G7157-87201 Needle High Flow (slotted), indicated by a white ring 5067-4277 2pos/6port Injection Valve, prep, 600 bar Reorder gives pack of 15 Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 192: Drawer Assembly

  Drawer for 50 x 2 mL Vials Classic Right G7129-68210 Drawer-Kit 100 x 2 mL Vials Classic Figure 22 Drawer for 66 x 2.0 mL Vials Figure 23 Drawer for 18 x 6 mL Vials Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 193
  It is required that all drawers are put into correct positions, that is, classical drawer 1-50 N O T E must be on the left side and classical drawer 51-100 on the right side. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 194: External Tray

  Parts and Materials for Maintenance External Tray External Tray Description G7129-60000 External Tray for 5 x 2 mL Vials G1313-27302 Disposal tube Figure 25 External tray Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 195: Analytical-Head Assembly (900 Μl)

  G7129-27790 Analytical-Head 900 µL 0905-1294 Metering seal, 900 µL 5001-3764 Support seal assembly, 900 µL G7129-60006 Adapter-Assembly Analytical-Head 0515-0850 Screws G4267-60462 Piston Assembly, 900 µL Figure 26 Analytical head assembly (900 µL) Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 196: Standard Sampler Accessory Kit

  CAN cable, Agilent module to module, 1 m G1313-44101 Finger Cap 5500-1155 Tube Connector, 90 degree, ID 6.4 5500-1254 Tube connector 180 ° 5500-1251 Capillary ST 0.12 mmX 400 mm SL/SL 5043-1013 Tubing Clip Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 197: Multi-Draw Kit

  The internal volume of the seat capillary is 400 µL or 1400 µL. With the sample loop the N O T E maximum draw volume of 500 µL or 1500 µL can be reached. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 198: Additional Part List

  Parts and Materials for Maintenance Additional Part List Additional Part List Description G7129-68001 Insulation Kit G7129-68000 Air guide kit G7129-60014 Calibration Tool 5023-2540 Adapter ICC Diffusor G7116-67110 MCT temperature verification kit G7129-40050 Transport-Protection G7129-20025 Gripper adapter Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 199: Identifying Cables

  Agilent 1260 Infinity II Preparative Autosampler User Manual Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables This chapter provides information on cables used with the Agilent InfinityLab LC Series modules. Agilent Technologies…

• Page 200: Cable Overview

  Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter…

• Page 201
  1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m USB cables Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 202: Analog Cables

  The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent Signal Name module Not connected Shield Analog — Center Analog + Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 203
  Pin Agilent Signal Name module Shield Shield Analog — Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog — Analog + Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 204: Remote Cables

  High grey NOT USED pink SHUT DOWN blue START PREPARE black 1wire DATA violet DGND grey-pink +5V ERI out red-blue PGND white-green PGND brown-green +24V ERI out white-yellow +24V ERI out yellow-brown Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 205
  • 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 206
  Ground Cable Shielding One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 207
  Wire Color Pin Agilent Signal Name Active module (TTL) White Digital ground Brown Prepare run Gray Start Blue Shut down Pink connected Yellow Power on High Ready High Green Stop Black Start request Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 208: Can/Lan Cables

  CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 209: Rs-232 Cables

  It’s also called «Null Modem Cable» with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 210: Usb

  To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 211: Hardware Information

  Agilent 1260 Infinity II Preparative Autosampler User Manual Hardware Information Firmware Description Electrical Connections Rear View of the Module Serial Number Information Interfaces Overview Interfaces ERI (Enhanced Remote Interface) USB (Universal Serial Bus) Setting the 6-bit Configuration Switch Special Settings…

• Page 212: Firmware Description

  • run synchronization through APG/ERI remote, • error handling, • diagnostic functions, • or module specific functions like • internal events such as lamp control, filter movements, • raw data collection and conversion to absorbance. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 213
  Update of main system can be done in the resident system only. Update of the resident N O T E system can be done in the main system only. Main and resident firmware must be from the same set. Figure 27 Firmware Update Mechanism Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 214
  All these specific informations are described in the documentation provided with the firmware update tools. The firmware update tools, firmware and documentation are available from the Agilent web. • http://www.agilent.com/en-us/firmwareDownload?whid=69761 Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 215: Electrical Connections

  There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.

• Page 216: Rear View Of The Module

  • CN = China Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module) 00000 Serial number Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 217: Interfaces

  Agilent InfinityLab LC Series Interfaces Module RS-232 Analog APG (A) Special (on-board) / ERI (E) Pumps G7104A/C G7110B G7111A/B, G5654A G7112B G7120A G7161A/B Samplers G7129A/B/C G7167B/C, G5667A G7157A Detectors G7114A/B G7115A G7117A/B/C G7121A/B G7162A/B G7165A Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 218
  • RS-232C as interface to a computer • USB (Universal Series Bus) as interface to a computer • REMOTE connector as interface to other Agilent products • Analog output connector(s) for signal output Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 219: Overview Interfaces

  Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 220
  All common TTL circuits operate with a 5 V power supply. A TTL signal is defined as «low» N O T E or L when between 0 V and 0.8 V and «high» or H when between 2.0 V and 5.0 V (with respect to the ground terminal). Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 221: Eri (Enhanced Remote Interface)

  1090/1040/1050/1100 HPLC systems and Agilent 1100/1200/1200 Infinity HPLC modules. All new InfinityLab LC Series products using the FUSION core electronics use ERI. This interface is already used in the Agilent Universal Interface Box 2 (UIB2) Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 222
  This way the interface can support various additional devices like sensors, triggers (in and out) and small controllers, etc. Figure 29 Location of the ERI interface (example shows a G7114A/B VWD) Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 223
  This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI-device. The firmware can detect the connected type of device automatically and update information in the device (if required). Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 224: Usb (Universal Serial Bus)

  • Short circuit will be detected through hardware. USB (Universal Serial Bus) USB (Universal Serial Bus) — replaces RS232, supports: • a PC with control software (for example Agilent Lab Advisor) • USB Flash Disk Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 225: Setting The 6-Bit Configuration Switch

  • For specific LAN modes switches 4-5 must be set as required. • For boot resident/cold start modes switches 1+2 or 6 must be UP. Figure 30 Location of Configuration switch (example shows a G7114A/B VWD) Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 226
  When selecting mode COM, settings are stored to non-volatile memory. When selecting mode TEST, COM settings are taken from non-volatile memory. not assigned — Always keep these switches on position ‘0’ (off) Default IP Address is 192.168.254.11 Host Name will be the MAC address. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 227: Special Settings

  ➔ Save your methods and data before executing a forced cold start. If you use the following switch settings and power the instrument up again, it will start as described above. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 228
  Boot Main System / Keep Data Boot Resident System / Keep Data Boot Main System / Revert to Default Data Boot Resident System / Revert to Default Data Note: The setting ‘0’ (down) is essential. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 229: Instrument Layout

  • the plastic layers help cushion the electronic and mechanical parts from physical shock, and • the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 230: Early Maintenance Feedback (Emf)

  EMF limits, and then reset the EMF counters to zero. The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 231
  Agilent 1260 Infinity II Preparative Autosampler User Manual LAN Configuration What You Have to Do First TCP/IP parameter configuration Configuration Switches Initialization Mode Selection Dynamic Host Configuration Protocol (DHCP) General Information (DHCP) Setup (DHCP) Manual Configuration With Telnet PC and Agilent ChemStation Setup…

• Page 232: Lan Configuration What You Have To Do First

  232) to • the PC network card using a crossover network cable (point-to-point) or • a hub or switch using a standard LAN cable. Figure 32 Location of LAN interfaces and MAC label Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 233: Tcp/Ip Parameter Configuration

  TCP/IP parameters after power-on. The parameters may be derived non-volatile memory or initialized with known default values. The initialization mode is selected by the configuration switch, see Table 17 page 235. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 234: Configuration Switches

  Location of Configuration switch (example shows a G7114A/B VWD) The module is shipped with all switches set to OFF, as shown above. To perform any LAN configuration, SW1 and SW2 must be set to OFF. N O T E Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 235: Initialization Mode Selection

  The TCP/IP connection will be established using these parameters. The parameters were configured previously by one of the described methods. Figure 34 Using Stored (Principle) Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 236
  In the Using Default mode, the parameters stored in the memory of the module are not N O T E cleared automatically. If not changed by the user, they are still available, when switching back to the mode Using Stored. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 237: Dynamic Host Configuration Protocol (Dhcp)

  2 It may be necessary to fully qualify the hostname with the DNS suffix, e.g. 0030d3177321.country.company.com. 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conventions. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 238: Setup (Dhcp)

  Card or the main board of above mentioned modules. Table 19 G1369C LAN Interface Card (configuration switch on the card) SW 4 SW 5 SW 6 SW 7 SW 8 Initialization Mode DHCP Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 239
  Lab Advisor, Firmware Update Tool) and use MAC address as host name, e.g. 0030d3177321. The LC system should become visible in the control software (see Note in section “General Information (DHCP)” on page 237). Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 240: Manual Configuration

  Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, given that the initialization mode selection switches are allowing Figure 38 Manual Configuration (Principle) Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 241: With Telnet

  When the connection was established successfully, the module responds with the following: Figure 40 A connection to the module is made 3 Type ? and press enter to see the available commands. Figure 41 Telnet Commands Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 242
  Initialization mode is Using Stored active TCP/IP settings TCP/IP status — here ready connected to PC with controller software (e.g. Agilent Figure 42 Telnet — Current settings in «Using Stored» mode ChemStation), here not connected Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 243
  If the Initialization Mode Switch is changed now to “Using Stored” mode, the instrument N O T E will take the stored settings when the module is re-booted. In the example above it would be 192.168.254.12. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 244: Pc And Agilent Chemstation Setup

  This procedure describes the change of the TCP/IP settings on your PC to match the module’s default parameters in a local configuration (see Table 18 on page 236). 1 Open the Local Area Connection Properties and select Internet Protocol (TCP/IP). Then click on Properties. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 245
  LAN Configuration PC and Agilent ChemStation Setup 2 You may enter here the fixed IP address of the module or use the Alternative Configuration. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 246
  LAN Configuration PC and Agilent ChemStation Setup 3 We will use the direct LAN access via Cross-over LAN cable with the module’s IP address. 4 Click on OK to save the configuration. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 247: Agilent Chemstation Setup

  N O T E Control Software. 1 Open the ChemStation Configuration Editor. 2 Select from the menu Configure — Instruments. 3 Select Modular 3D LC System. 4 Give the Instrument a name. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 248
  LAN Configuration PC and Agilent ChemStation Setup 5 Click on OK. 6 Select LC System Access — Access Point and click on Add. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 249
  9 Save the current configuration via File – Save. 10 Exit the Configuration Editor. 11 Start the Agilent ChemStation. During first startup or when the system configuration has changed, a notification shows up. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 250
  14 Click on OK. The selected module is shown now in the right window (with serial number). In addition all other modules connected via CAN to the detector are shown as well. Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 251
  Under Connection Settings you may change the IP/Hostname of the module (may require a re-start of the ChemStation). After successful load of the ChemStation, you should see the module(s) as active item in the graphical user interface (GUI). Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 252
  LAN Configuration PC and Agilent ChemStation Setup Figure 45 Screen After Successful Load of ChemStation Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 253: Appendix

  Agilent 1260 Infinity II Preparative Autosampler User Manual Appendix General Safety Information General Safety Information Safety Standards General Before Applying Power Ground the Instrument Ground Solvent Lines Do Not Operate in an Explosive Atmosphere Do Not Remove the Instrument Cover…

• Page 254: General Safety Information

  Do not use this product in any manner not specified by the manufacturer. The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 255: Before Applying Power

  Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. ➔…

• Page 256: Ground Solvent Lines

  Do not install substitute parts or perform any unauthorized modification to the product. Return the product to an Agilent Sales and Service Office for service and repair to ensure that safety features are maintained. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 257: In Case Of Damage

  Personal injury (for example electrical shock, intoxication) ➔ Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 258: Solvents

  For details, see the usage guideline for the solvent cabinet. A printed copy of the guideline N O T E has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 259: Symbols

  A pacemaker could switch into test mode and cause illness. A heart defibrillator may stop working. If you wear these devices keep at least 55 mm distance to magnets. Warn others who wear these devices from getting too close to magnets. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 260
  C AU T I O N alerts you to situations that could cause loss of data, or damage of equipment. ➔ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 261: Waste Electrical And Electronic Equipment Directive

  Monitoring and Control Instrumentation product. Do not dispose of in domestic household waste N O T E To return unwanted products, contact your local Agilent office, or see http://www.agilent.com for more information. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 262: Refrigerant

  The refrigerant HFC-134a is used only in the Agilent Infinity II Sample Cooler. Table 23 Physical properties of refrigerant HFC-134a Molecular weight Critical temperature 101.1 °C Critical pressure 40.6 bar Boiling point -26.5 °C Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 263
  All media must be disposed of in accordance with national and local regulations. ➔ Please contact your local Agilent Service Center in regard to safe environmental disposal of the appliance or check www.agilent.com for more info. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 264: Radio Interference

  Appendix Radio Interference Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with equipment unscreened…

• Page 265: Sound Emission

  This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 266: Solvent Information

  (e.g. EDTA), • mixtures of carbon tetrachloride with 2-propanol or THF. • Avoid the use of dimethyl formamide (DMF). Polyvinylidene fluoride (PVDF), which is used in leak sensors, is not resistant to DMF. Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 267: Agilent Technologies On Internet

  Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Agilent 1260 Infinity II Preparative Autosampler User Manual…

• Page 268
  104, cable exchange DRAW 58, 104, capillaries drawers drawer caps drawers crimp exchange screw fan failed snap features change metering seal 113, G7157A eject speed 104, change needle function Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 269
  Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 270
  X-axis replacing firmware reset RS-232C TCP/IP parameter configuration cable Z-axis telnet configuration theta-axis safety class I timeout safety transport assembly general information transport standards troubleshooting Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 271
  Index Agilent 1260 Infinity II Preparative Autosampler User Manual…
• Page 272
  In This Book This manual contains technical reference information about the Agilent 1260 Infinity II Preparative Autosampler (G7157A). The manual describes the following: • Introduction, • site requirements and specifications, • using the module, • optimizing performance, • troubleshooting and diagnostics, •…

1-канальный изократический насос Agilent 1260 Infinity II

Характеристики: обеспечение потока от 0,001 до 10 мл/мин (с шагом 0,001 мл/мин); создаваемое давление от 0 до 600 бар; погрешность воспроизводимости потока ≤0,07 % ОСО; точность задания потока ±1%.

В комплекте с насосом поставляются: тестовая ВЭЖХ колонка C18, капилляры, поддон для бутылей, четыре 1 л бутыли и кабели для подключения.

В качестве дополнительных опций к насосу можно добавить: набор инструментов для обслуживания, комплект расходных материалов, встроенный 2-канальный дегазатор с краном выбора до 3 растворителей.

2-канальный бинарный насос Agilent 1260 Infinity II

Характеристики: формирование градиента путем смешивания до 2 компонентов подвижной фазы в зоне высокого давления; максимально высокая воспроизводимость расхода и смешивания подвижной фазы; создаваемое давление от 0 до 600 бар; обеспечение потока от 0,001 до 5 мл/мин (с шагом 0,001 мл/мин); встроенный 2-канальный дегазатор для удаления газов из подвижной фазы; погрешность воспроизводимости потока ≤0,07 % ОСО; градиентный диапазон 0-100%; погрешность воспроизводимости состава градиента <0,15% ОСО; точность задания потока ±1%; рабочий диапазон pH от 1 до 12,5.

В комплекте с насосом поставляются: тестовая ВЭЖХ колонка C18, капилляры, поддон для бутылей, четыре 1 л бутыли и кабели для подключения.

В качестве дополнительных опций к насосу можно добавить: набор инструментов для обслуживания, комплект расходных материалов, кран выбора растворителей, встроенную систему автоматической промывки уплотнителей Active Seal Wash, заменить пассивный клапан (PIV) на активный (AIV).

4-канальный квартернарный насос Agilent 1260 Infinity II

Характеристики: формирование градиента путем смешивания до 4 компонентов подвижной фазы в зоне низкого давления; максимально высокая воспроизводимость расхода и смешивания подвижной фазы; создаваемое давление от 0 до 400 (насос VL) или 600 бар; встроенный 4-канальный дегазатор для удаления газов из подвижной фазы; обеспечение потока от 0,001 до 10 мл/мин (с шагом 0,001 мл/мин); погрешность воспроизводимости потока ≤0,07 % ОСО; точность задания потока ±1%; рабочий диапазон pH от 1 до 12,5.

В комплекте с насосом поставляются: тестовая ВЭЖХ колонка C18, капилляры, поддон для бутылей, четыре 1 л бутыли и кабели для подключения.

В качестве дополнительных опций к насосу можно добавить: набор инструментов для обслуживания, комплект расходных материалов, встроенную систему автоматической промывки уплотнителей Active Seal Wash, заменить пассивный клапан (PIV) на активный (AIV).

4-канальный квартернарный насос Agilent 1260 Infinity II Prime

Характеристики: ВЭЖХ насос на технологии СВЭЖХ системы 1290 II; формирование градиента путем смешивания до 4 компонентов подвижной фазы в зоне низкого давления; максимально высокая воспроизводимость расхода и смешивания подвижной фазы; создаваемое давление от 0 до 800 бар; встроенный 4-канальный дегазатор для удаления газов из подвижной фазы; встроенная система автоматической промывки уплотнителей Active Seal Wash; обеспечение потока от 0,001 до 5 мл/мин (с шагом 0,001 мл/мин); погрешность воспроизводимости потока ≤0,07 % ОСО; точность задания потока ±1%; градиентный диапазон 0-100%; погрешность воспроизводимости состава градиента <0,15% ОСО; рабочий диапазон pH от 1 до 12,5; «мертвый» объем ≤ 350 мкл.

В комплекте с насосом поставляются: тестовая ВЭЖХ колонка C18, капилляры, поддон для бутылей, четыре 1 л бутыли и кабели для подключения.

В качестве дополнительных опций к насосу можно добавить: набор инструментов для обслуживания, комплект расходных материалов, набор капилляров для масс-детектора, микропотоковую систему смешения Jet Weaver и систему эмуляции ISET.

Анализ органических загрязняющих веществ в воде методом ВЭЖХ с онлайн-ТФЭ

Скрининговое исследование 36 ветеринарных лекарственных препаратов в пищевых продуктах животного происхождения методом ЖХ-МСМС в сочетании с QuEChERS

Многокомпонентный скрининг и количественное определение пестицидов в сложных матрицах с применением трехквадрупольного Agilent 6495

Определение водорастворимых витаминов в пищевых продуктах методом ВЭЖХ с МСД

Определение сорбиновой и бензойной кислоты в маслах и спредах методом микроэмульсионныой жидкостной хроматографии

Анализ продуктов питания

Определение водорастворимых витаминов в витаминных премиксах, БАД-ах и фармацевтических препаратах методом ВЭЖХ с градиентным элюированием

Руководство по методам контроля качества и безопасности биологически активных добавок к пище

ГОСТ 31669-2012 Продукция соковая. Определение сахарозы, глюкозы, фруктозы и сорбита методом высокоэффективной жидкостной хроматографии

ГОСТ 30059-93 Методы определения аспартама, сахарина, кофеина и бензоата натрия

ГОСТ 32903-2014 Продукция соковая. Определение водорастворимых витаминов: тиамина (B(1)), рибофлавина (В(2)), пиридоксина (B(6)) и никотинамида (PP) методом обращенно-фазовой высокоэффективной жидкостной хроматографии

ГОСТ EN 12856-2015 Продукция пищевая. Определение ацесульфама калия, аспартама и сахарина методом ВЭЖХ

ГОСТ Р 52060-2003 Патока крахмальная. Общие технические условия

Determination of 24 PAHs in Drinking water

ГОСТ 31860-2012 Вода питьевая. Метод определения содержания бенз(а)пирена

ПНД Ф 14.1:2:4.70-96 Количественный химический анализ вод. Методика измерений массовых концентраций ПАУ в питьевых, природных и сточных водах методом ВЭЖХ

ГОСТ 32903-2014 Продукция соковая. Определение водорастворимых витаминов: тиамина (B(1)), рибофлавина (В(2)), пиридоксина (B(6)) и никотинамида (PP) методом обращенно-фазовой высокоэффективной жидкостной хроматографии

ГОСТ 32771-2014 Продукция соковая. Определение органических кислот методом обращенно-фазовой высокоэффективной жидкостной хроматографии

ГОСТ 32712-2014 Продукция соковая. Определение фумаровой кислоты методом высокоэффективной жидкостной хроматографии

ГОСТ 32709-2014 Продукция соковая. Методы определения антоцианинов

ГОСТ Р 54744-2011 Продукция соковая. Определение хинной, яблочной и лимонной кислот в продуктах из клюквы и яблок методом высокоэффективной жидкостной хроматографии

ГОСТ Р 54742-2011 Продукция соковая. Определение нарингина и неогесперидина в апельсиновом соке методом высокоэффективной жидкостной хроматографии

ГОСТ 31669-2012 Продукция соковая. Определение сахарозы, глюкозы, фруктозы и сорбита методом высокоэффективной жидкостной хроматографии

ГОСТ 31644-2012 Продукция соковая. Определение 5-гидроксиметилфурфурола методом высокоэффективной жидкостной хроматографии (с Изменением N 1)

ГОСТ 31643-2012 Продукция соковая. Определение аскорбиновой кислоты методом высокоэффективной жидкостной хроматографии

ГОСТ Р 51435-99 (ИСО 8128-1-93) Сок яблочный, сок яблочный концентрированный и напитки, содержащие яблочный сок. Метод определения содержания патулина с помощью высокоэффективной жидкостной хроматографии

ГОСТ Р 51428-99 Соки фруктовые. Метод определения содержания винной кислоты с помощью высокоэффективной жидкостной хроматографии

ГОСТ Р 51427-99 Соки цитрусовые. Метод определения массовой концентрации гесперидина и нарингина с помощью высокоэффективной жидкостной хроматографии

МУК 4.1.1912-04 Определение остаточных количеств левомицетина (Хлорамфеникола, Хлормецитина) в продуктах животного происхождения методом высокоэффективной жидкостной хроматографии и иммуноферментного анализа

ГОСТ 32307-2013 Мясо и мясные продукты. Определение содержания жирорастворимых витаминов методом высокоэффективной жидкостной хроматографии

ГОСТ ISO 13493-2014 Мясо и мясные продукты. Метод определения содержания хлорамфеникола (левомицетина) с помощью жидкостной хроматографии

МУК 4.1.1012-01 Определение массовой концентрации аверсектина С в органах и тканях животных, плазме и молоке методом флуоресцентной высокоэффективной жидкостной хроматографии

ГОСТ 32251-2013 (ISO 17375:2006) Корма, комбикорма. Метод определения содержания афлатоксина В1

ГОСТ 32201-2013 (ISO 13904:2005) Корма, комбикорма. Метод определения содержания триптофана

ГОСТ 33616-2015 Продукты пищевые, продовольственное сырье. Метод определения остаточного содержания мышьяксодержащих стимуляторов роста с помощью ВЭЖХ-ИСП-МС

ГОСТ 32835-2014 Продукция соковая. Определение микотоксинов методом тандемной высокоэффективной жидкостной хроматомасс-спектрометрии (ВЭЖХ-МС/МС)

ГОСТ 32195-2013 (ISO 13903:2005) Корма, комбикорма. Метод определения содержания аминокислот

ГОСТ Р 54950-2012 (ИСО 14565:2000) Корма для животных. Определение содержания витамина А методом высокоэффективной жидкостной хроматографии

ГОСТ Р 54949-2012 (ИСО 6867:2000) Корма для животных. Определение содержания витамина Е методом высокоэффективной жидкостной хроматографии

ГОСТ Р 55448-2013 Корма, комбикорма, комбикормовое сырье. Определение содержания охратоксина А методом высокоэффективной жидкостной хроматографии с флуориметрическим детектированием

ГОСТ 32587-2013 Зерно и продукты его переработки, комбикорма. Определение охратоксина А методом высокоэффективной жидкостной хроматографии

ГОСТ 31691-2012 Зерно и продукты его переработки, комбикорма. Определение содержания зеараленона методом высокоэффективной жидкостной хроматографии (с Поправкой)

ГОСТ 31789-2012 Рыба, морские беспозвоночные и продукты их переработки. Количественное определение содержания биогенных аминов методом высокоэффективной жидкостной хроматографии

ГОСТ Р 51116-97 Комбикорма, зерно, продукты его переработки. Метод определения содержания дезоксиниваленола (вомитоксина)

ГОСТ 26573.1-93 Премиксы. Методы определения витамина А

ГОСТ 32043-2012 Премиксы. Методы определения витаминов А, D, Е

ГОСТ 32042-2012 Премиксы. Методы определения витаминов группы В

ГОСТ 32258-2013 Молоко и молочная продукция. Метод определения массовой доли бенз(а)пирена

ГОСТ Р 55482-2013 Мясо и мясные продукты. Метод определения содержания водорастворимых витаминов

ГОСТ 31504-2012 Молоко и молочная продукция. Определение содержания консервантов и красителей методом высокоэффективной жидкостной хроматографии

ГОСТ Р 54760-2011 Продукты молочные составные и продукты детского питания на молочной основе. Определение массовой концентрации моно- и дисахаридов методом высокоэффективной жидкостной хроматографии

ГОСТ ISO/TS 15495/IDF/RM 230-2012 Молоко. Молочные продукты и питание для детей раннего возраста. Руководящие указания для количественного определения меламина и циануровой кислоты методом жидкостной хроматографии — тандемной масс-спектрометрии (LC-MS/MS)

Р 4.1.1672-03 Руководство по методам контроля качества и безопасности биологически активных добавок к пище

ГОСТ 28038-2013 Продукты переработки плодов и овощей. Методы определения микотоксина патулина (с Поправкой)

ГОСТ 32123-2013 (ISO 15302:2007) Жиры и масла животные и растительные. Определение содержания бенз(а)пирена. Метод с применением высокоразрешающей жидкостной хроматографии с обратной фазой

ГОСТ 31745-2012 Продукты пищевые. Определение содержания полициклических ароматических углеводородов методом высокоэффективной жидкостной хроматографии

ГОСТ 31748-2012 (ISO 16050:2003) Продукты пищевые. Определение афлатоксина B(1) и общего содержания афлатоксинов B(1), B(2), G(1) и G(2) в зерновых культурах, орехах и продуктах их переработки. Метод высокоэффективной жидкостной хроматографии (с Поправкам

ГОСТ 31768-2012 Мед натуральный. Методы определения гидроксиметилфурфураля

ГОСТ Р ИСО 9233-2-2011 Сыры и плавленые сыры. Определение содержания натамицина. Часть 2. Метод высокоэффективной жидкостной хроматографии

ГОСТ Р 54637-2011 Продукты пищевые функциональные. Метод определения витамина D(3)

ГОСТ Р ЕН 14130-2010 Продукты пищевые. Определение витамина С с помощью высокоэффективной жидкостной хроматографии

ГОСТ EN 12857-2015 Продукция пищевая. Определение цикламата методом высокоэффективной жидкостной хроматографии

ГОСТ EN 12856-2015 Продукция пищевая. Определение ацесульфама калия, аспартама и сахарина методом высокоэффективной жидкостной хроматографии

ГОСТ 32167-2013 Мед. Метод определения сахаров

ГОСТ 31762-2012 Майонезы и соусы майонезные. Правила приемки и методы испытаний (с Поправками, с Изменением N 1)

ГОСТ 30711-2001 Продукты пищевые. Методы выявления и определения содержания афлатоксинов В(1) и М(1)

ГОСТ Р 51650-2000 Продукты пищевые. Методы определения массовой доли бенз(а)пирена

МУ 4082-86 Методика определения афлатоксинов в пищевых продуктах с помощью высокоэффективной жидкостной хроматографии

ГОСТ Р 54904-2012 Продукты пищевые, продовольственное сырье. Метод определения остаточного содержания сульфаниламидов, нитроимидазолов, пенициллинов, амфениколов с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектором (с

ГОСТ 31694-2012 Продукты пищевые, продовольственное сырье. Метод определения остаточного содержания антибиотиков тетрациклиновой группы с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектором (с Изменением N 1, с Поправко

ГОСТ Р 54518-2011 Продукты пищевые, корма, продовольственное сырье. Метод определения содержания кокцидиостатиков с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектором (с Поправкой)

ГОСТ 32014-2012 Продукты пищевые, продовольственное сырье. Метод определения остаточного содержания метаболитов нитрофуранов с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектором (с Изменением N 1, с Поправкой)

МУК 4.1.2229-07 Определение домоевой кислоты в морепродуктах методом высокоэффективной жидкостной хроматографии

ГОСТ ISO 10727-2013 Чай и чай растворимый. Определение содержания кофеина. Метод высокоэффективной жидкостной хроматографии

ГОСТ EN 14132-2013 Продукты пищевые. Определение охратоксина А в ячмене и жареном кофе. Метод ВЭЖХ с применением иммуноаффинной колоночной очистки экстракта

ГОСТ ISO 20481-2013 Кофе и кофейные продукты. Определение содержания кофеина с использованием высокоэффективной жидкостной хроматографии (HPLC). Стандартный метод

МУК 4.1.1234-03 Определение остаточных количеств Фенамидона и его метаболитов (RPA 405862 и RPA 408056) в воде, почве, картофеле, томатах, луке и огурцах методом высокоэффективной жидкостной хроматографии

МУК 4.1.1215-03 Определение остаточных количеств Амидосульфурона в воде, почве, зерне и соломе зерновых колосовых культур, зерне и зеленой массе кукурузы методом высокоэффективной жидкостной хроматографии

МУК 4.1.1213-03 Определение остаточных количеств Азоксистробина (ICI A 5504) и его геометрического изомера (R-230310) в воде, почве, в плодах огурцов, томатов, ягодах винограда, в зерне и соломе зерновых колосовых культур методом высокоэффективной жидкост

МУК 4.1.1148-02 Определение остаточных количеств Флудиоксонила в воде, почве, зеленой массе растений, клубнях картофеля, зерне и соломе хлебных злаков, зерне кукурузы, семенах и масле подсолнечника методом высокоэффективной жидкостной хроматографии

МУК 4.1.2332-08 Определение остаточных количеств флудиоксонила в семенах и масле рапса, ягодах и соке винограда методом высокоэффективной жидкостной хроматографии

МУК 4.1.2055-06 Методические указания по определению остаточных количеств флудиоксонила в зерне и масле сои методом высокоэффективной жидкостной хроматографии

МУК 4.1.1146-02 Определение остаточных количеств Фамоксадона в воде, почве, клубнях картофеля, зеленой массе, соломе и зерне зерновых колосовых культур методом высокоэффективной жидкостной хроматографии

ГОСТ 33809-2016 Мясо и мясные продукты. Определение сорбиновой и бензойной кислот методом высокоэффективной жидкостной хроматографии

ГОСТ 34228-2017 Продукция соковая. Определение консервантов методом высокоэффективной жидкостной хроматографии

ГОСТ 34456-2018 Молоко и продукция молочная. Определение состава стеринов методом высокоэффективной жидкостной хроматографии

ГОСТ 34230-2017 Продукция соковая. Определение свободных аминокислот методом высокоэффективной жидкостной хроматографии

ГОСТ 33526-2015 Молоко и продукты переработки молока. Методика определения содержания антибиотиков методом высокоэффективной жидкостной хроматографии

ГОСТ ISO 16931-2014 Жиры и масла животные и растительные. Определение содержания полимеризованных триацилглицеринов методом высокоэффективной эксклюзионной хроматографии (ВЭЭХ)

ГОСТ 34151-2017 Продукты пищевые. Определение витамина С с помощью высокоэффективной жидкостной хроматографии

ГОСТ 34133-2017 Мясо и мясные продукты. Метод определения аскорбиновой кислоты и аскорбатов высокоэффективной жидкостной хроматографией

ГОСТ 33780-2016 Продукты пищевые, корма, комбикорма. Определение содержания афлатоксина В1 методом высокоэффективной жидкостной хроматографии с применением очистки на оксиде алюминия

ГОСТ 33409-2015 Продукция алкогольная и соковая. Определение содержания углеводов и глицерина методом высокоэффективной жидкостной хроматографии

ГОСТ 33460-2015 Продукция соковая. Определение ксилита, сорбита и маннита методом высокоэффективной жидкостной хроматографии

ГОСТ 34119-2017 Мясо и мясные продукты. Метод определения полициклических ароматических углеводородов высокоэффективной жидкостной хроматографией с масс-спектрометрическим детектированием

ГОСТ 33607-2015 Мясо и мясные продукты. Определение бета-агонистов методом высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектором

ГОСТ 33422-2015 Мясо и мясные продукты. Определение массовой доли йодтирозинов методом высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектором

ГОСТ 33429-2015 Мясо и мясные продукты. Определение содержания молочной кислоты и лактатов методом высокоэффективной жидкостной хроматографии

ГОСТ 33934-2016 Мясо и мясные продукты. Определение цинкбацитрацина методом высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектором

ГОСТ 33808-2016 Мясо и мясные продукты. Определение лимонной кислоты методом высокоэффективной жидкостной хроматографии

ГОСТ 34119-2017 Мясо и мясные продукты. Метод определения полициклических ароматических углеводородов высокоэффективной жидкостной хроматографией с масс-спектрометрическим детектированием

ГОСТ 34480-2018 Мясо и мясные продукты. Определение амфениколов и пенициллинов методом тандемной жидкостной масс-спектрометрии

ГОСТ 34136-2017 Продукты пищевые, продовольственное сырье. Метод определения остаточного содержания макролидов, линкозамидов и плевромутилинов с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием

ГОСТ 32797-2014 Продукты пищевые, продовольственное сырье. Метод определения остаточного содержания хинолонов с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектором (с Поправками)

ГОСТ 34137-2017 Продукты пищевые, продовольственное сырье. Метод определения остаточного содержания цефалоспоринов с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием

ГОСТ 32798-2014 Продукты пищевые, продовольственное сырье. Метод определения остаточного содержания аминогликозидов с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектором (с Поправками)

ГОСТ 33482-2015 Продукты пищевые, сырье продовольственное, комбикорма. Метод определения содержания анаболических стероидов и производных стильбена с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием (с Поправкой

ГОСТ 32834-2014 Продукты пищевые, продовольственное сырье. Метод определения остаточного содержания антгельминтиков с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектором (с Поправками)

ГОСТ 33971-2016 Продукты пищевые, продовольственное сырье. Метод определения остаточного содержания метаболитов карбадокса и олаквиндокса с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектором (с Поправкой)

ГОСТ 33978-2016 Продукты пищевые и комбикорма. Метод определения содержания тиреостатиков с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием

ГОСТ 34139-2017 Продукты пищевые, продовольственное сырье. Метод определения остаточного содержания седативных препаратов и адреноблокаторов с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием

ГОСТ 34140-2017 Продукты пищевые, корма, продовольственное сырье. Метод определения микотоксинов с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием

ГОСТ 34461-2018 Продукция соковая. Определение содержания гесперидина и нарингина методом высокоэффективной жидкостной хроматографии

ГОСТ 34439-2018 Средства лекарственные для ветеринарного применения, корма, кормовые добавки. Определение содержания антиоксидантов методом высокоэффективной жидкостной хроматографии со спектрофотометрическим детектированием

ФР.1.31.2008.04632 Методика выполнения измерений массовой доли лизина, триптофана, метионина, суммы цистина и цистеина в комбикормах, премиксах и комбикормовом сырье методом высокоэффективной жидкостной хроматографии

ГОСТ 34616-2019 Продукты пищевые, продовольственное сырье. Определение содержания полициклических ароматических углеводородов методом высокоэффективной жидкостной хроматографии с флуориметрическим детектированием (с Поправкой)

ГОСТ 34049-2017 Молоко и кисломолочные продукты. Определение содержания афлатоксина М(1) методом высокоэффективной жидкостной хроматографии с флуориметрическим (спектрофлуориметрическим) детектированием

ГОСТ ISO 9231-2015 Молоко и молочные продукты. Определение содержания сорбиновой и бензойной кислот в молоке и молочных продуктах (Переиздание)

Анализ органических загрязняющих веществ в воде методом ВЭЖХ с онлайн-ТФЭ

Analysis of Polycyclic Aromatic Hydrocarbons in Soil with Agilent Bond Elut HPLC-FLD

Analysis of PAHs in soil according to EPA 8310 method with UV and fluorescence detection

Determination of 24 PAHs in Drinking water

ГОСТ 31860-2012 Вода питьевая. Метод определения содержания бенз(а)пирена

ПНД Ф 14.1:2:4.70-96 Количественный химический анализ вод. Методика измерений массовых концентраций ПАУ в питьевых, природных и сточных водах методом ВЭЖХ

ГОСТ Р ИСО 16362-2009 Воздух атмосферный. Определение содержания полициклических ароматических углеводородов в виде твердых частиц методом высокоэффективной жидкостной хроматографии

ПНД Ф 16.1:2.2:2.3:3.62-09 Количественный химический анализ почв. Методика выполнения измерений массовых долей ПАУ в почвах, донных отложения, осадках сточных вод и отходах производства и потребления методом ВЭЖХ

ФР.1.31.2004.01279 Методика выполнения измерений массовой доли полиароматических углеводородов в пробах почв и донных отложений методом высокоэффективной жидкостной хроматографии

МУК 4.1.1234-03 Определение остаточных количеств Фенамидона и его метаболитов (RPA 405862 и RPA 408056) в воде, почве, картофеле, томатах, луке и огурцах методом высокоэффективной жидкостной хроматографии

МУК 4.1.1215-03 Определение остаточных количеств Амидосульфурона в воде, почве, зерне и соломе зерновых колосовых культур, зерне и зеленой массе кукурузы методом высокоэффективной жидкостной хроматографии

МУК 4.1.1213-03 Определение остаточных количеств Азоксистробина (ICI A 5504) и его геометрического изомера (R-230310) в воде, почве, в плодах огурцов, томатов, ягодах винограда, в зерне и соломе зерновых колосовых культур методом высокоэффективной жидкост

МУК 4.1.1148-02 Определение остаточных количеств Флудиоксонила в воде, почве, зеленой массе растений, клубнях картофеля, зерне и соломе хлебных злаков, зерне кукурузы, семенах и масле подсолнечника методом высокоэффективной жидкостной хроматографии

МУК 4.1.1146-02 Определение остаточных количеств Фамоксадона в воде, почве, клубнях картофеля, зеленой массе, соломе и зерне зерновых колосовых культур методом высокоэффективной жидкостной хроматографии

ГОСТ Р 55227-2012 Вода. Методы определения содержания формальдегида

ГОСТ Р ИСО 17736-2013 Воздух рабочей зоны. Определение изоцианатов в воздухе с применением устройства отбора проб с двумя фильтрами и высокоэффективной жидкостной хроматографии

ГОСТ Р ИСО 17735-2012 Воздух рабочей зоны. Определение суммарного содержания изоцианатных групп в воздухе методом жидкостной хроматографии с использованием в качестве реагента 1-(9-антраценилметил)пиперазина (МАР)

ГОСТ Р ИСО 16702-2008 Качество воздуха рабочей зоны. Определение общего содержания изоцианатных групп органических соединений в воздухе методом жидкостной хроматографии с использованием 1-(2-метокси-фенил)пиперазина

МУК 4.1.1045-01 ВЭЖХ определение формальдегида и предельных альдегидов (С(2)-С(10)) в воздухе

МУК 4.1.1045а-01 ВЭЖХ определение диоксацина в воздухе

МУК 4.1.0.416-96 Измерение концентраций витамина В(1) методом высокоэффективной жидкостной хроматографии (ВЭЖХ) в воздухе рабочей зоны

МУК 4.1.0.398-96 Измерение концентраций фенигидина (2,6-диметил-3,5-диметоксикарбонил-4-(0-нитрофенил)-1,4-дигидропиридин) методом высокоэффективной жидкостной хроматографии в воздухе рабочей зоны

МУК 4.1.0.397-96 Измерение концентраций 3-три-фторметилацетанилида методом высокоэффективной жидкостной хроматографии в воздухе рабочей зоны

МУК 4.1.258-96 Измерение концентраций триметилсульфония бромистого методом высокоэффективной жидкостной хроматографии в воздухе рабочей зоны

МУК 4.1.243-96 Определение концентрации метиоприла в воздухе рабочей зоны методом ВЭЖХ

МУК 4.1.226-96 Измерение концентраций 3,5-дихлорсульфаниламида методом высокоэффективной жидкостной хроматографии в воздухе рабочей зоны

МУК 4.1.1274-03 Методы контроля. Химические факторы. Измерение массовой доли бенз(а)пирена в пробах почв, грунтов, донных отложений и твердых отходов методом ВЭЖХ с использованием флуориметрического детектора

ГОСТ 32533-2013 Гексаметилендиамин. Определение содержания в воздушной среде

ГОСТ Р ИСО 17734-1-2009 Анализ азоторганических соединений в воздухе методом жидкостной хроматографии и масс-спектрометрии. Часть 1. Определение изоцианатов по их дибутиламиновым производным

ГОСТ Р ИСО 11338-2-2008 Выбросы стационарных источников. Определение содержания полициклических ароматических углеводородов в газообразном состоянии и в виде твердых взвешенных частиц. Часть 2. Подготовка, очистка и анализ проб

МУК 4.1.1415-03 Определение кломазона в воде хроматографическими методами

Методика измерений массовой доли бенз(а)пирена в пробах почв, грунтов, твердых отходов, донных отложений, осадках сточных вод методом высокоэффективной жидкостной хроматографии с флуоресцентным детектированием жидкостного хроматографа «Люмахром»

Скрининговое исследование 36 ветеринарных лекарственных препаратов в пищевых продуктах животного происхождения методом ЖХ-МСМС в сочетании с QuEChERS

Определение водорастворимых витаминов в витаминных премиксах, БАД-ах и фармацевтических препаратах методом ВЭЖХ с градиентным элюированием

Determination of 24 PAHs in Drinking water

ГОСТ 32251-2013 (ISO 17375:2006) Корма, комбикорма. Метод определения содержания афлатоксина В1

ГОСТ 32201-2013 (ISO 13904:2005) Корма, комбикорма. Метод определения содержания триптофана

ГОСТ 32195-2013 (ISO 13903:2005) Корма, комбикорма. Метод определения содержания аминокислот

ГОСТ Р 54950-2012 (ИСО 14565:2000) Корма для животных. Определение содержания витамина А методом высокоэффективной жидкостной хроматографии

ГОСТ Р 54949-2012 (ИСО 6867:2000) Корма для животных. Определение содержания витамина Е методом высокоэффективной жидкостной хроматографии

ГОСТ Р 55448-2013 Корма, комбикорма, комбикормовое сырье. Определение содержания охратоксина А методом высокоэффективной жидкостной хроматографии с флуориметрическим детектированием

ГОСТ 32587-2013 Зерно и продукты его переработки, комбикорма. Определение охратоксина А методом высокоэффективной жидкостной хроматографии

ГОСТ 31691-2012 Зерно и продукты его переработки, комбикорма. Определение содержания зеараленона методом высокоэффективной жидкостной хроматографии (с Поправкой)

ГОСТ Р 51116-97 Комбикорма, зерно, продукты его переработки. Метод определения содержания дезоксиниваленола (вомитоксина)

ГОСТ 26573.1-93 Премиксы. Методы определения витамина А

ГОСТ 32043-2012 Премиксы. Методы определения витаминов А, D, Е

ГОСТ 32042-2012 Премиксы. Методы определения витаминов группы В

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МУК 4.1.1148-02 Определение остаточных количеств Флудиоксонила в воде, почве, зеленой массе растений, клубнях картофеля, зерне и соломе хлебных злаков, зерне кукурузы, семенах и масле подсолнечника методом высокоэффективной жидкостной хроматографии

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ГОСТ 33780-2016 Продукты пищевые, корма, комбикорма. Определение содержания афлатоксина В1 методом высокоэффективной жидкостной хроматографии с применением очистки на оксиде алюминия

ГОСТ ISO 15141-2-2013 Продукты пищевые. Определение содержания охратоксина А в зерне и зерновых продуктах. Часть 2. Метод высокоэффективной жидкостной хроматографии с очисткой бикарбонатом

ГОСТ 33482-2015 Продукты пищевые, сырье продовольственное, комбикорма. Метод определения содержания анаболических стероидов и производных стильбена с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием (с Поправкой

ГОСТ 33978-2016 Продукты пищевые и комбикорма. Метод определения содержания тиреостатиков с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием

ГОСТ 34140-2017 Продукты пищевые, корма, продовольственное сырье. Метод определения микотоксинов с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием

ГОСТ 34439-2018 Средства лекарственные для ветеринарного применения, корма, кормовые добавки. Определение содержания антиоксидантов методом высокоэффективной жидкостной хроматографии со спектрофотометрическим детектированием

ФР.1.31.2008.04632 Методика выполнения измерений массовой доли лизина, триптофана, метионина, суммы цистина и цистеина в комбикормах, премиксах и комбикормовом сырье методом высокоэффективной жидкостной хроматографии

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• Жидкостный хроматограф Agilent 1260 — высокотехнологичный специализированный прибор, созданный на основе системы 1200, обеспечивающий высокую скорость транспорта данных и помогающий в скорейшем достижении успешного завершения анализа.

• Компания лицензирована ФСБ

• Сервисное обслуживание

• Подбор и оснащение лабораторий

• Доставка

• Организация работы фармацевтической
  промышленности

Бинарный насос Agilent 1290 Infinity (БН-A1290I) — Скорость потока

Четырёхканальный насос Agilent 1290 Infinity (ЧН-A1290I) — Скорость потока

(ЧН-A1290I) Воспроизводимость потока, RSD

(ЧН-A1290I) Точность потока

(ЧН-A1290I) Диапазон давлений

(ЧН-A1290I) Пульсация давления

(ЧН-A1290I) Градиент

Изократический насос Agilent 1260 Infinity (ИН-A1260I) — Скорость потока

(ИН-A1260I) Воспроизводимость потока RSD

(ИН-A1260I) Точность потока

(ИН-A1260I) Диапазон давлений

(БН-A1260I) Воспроизводимость потока, RSD

(БН-A1260I) Точность потока

(БН-A1260I) Диапазон давлений

(БН-A1260I) Пульсация давления

(БН-A1260I) Градиент

Четырёхканальный биоинертный насос Agilent 1260 Infinity (ЧБН-A1260I) — Скорость потока

(ЧБН-A1260I) Воспроизводимость потока, RSD

(ЧБН-A1260I) Точность потока

(ЧБН-A1260I) Диапазон давлений

(ЧБН-A1260I) Пульсация давления

(ЧБН-A1260I) Градиент

(ЧБН-A1260I) Воспроизводимость потока, RSD

(ЧБН-A1260I) Смешение

Капиллярный насос Agilent 1260 Infinity (КН-A1260I) — Скорость потока

(КН-A1260I) Воспроизводимость потока, RSD

(КН-A1260I) Диапазон давлений

(КН-A1260I) Воспроизводимость смешения, RSD

(КН-A1260I) Диапазон

Нанопотоковый насос Agilent 1260 Infinity (НН-A1260I) — Скорость потока

(НН-A1260I) Градиент

(НН-A1260I) Диапазон давлений

(НН-A1260I) Воспроизводимость смешения, RSD

(НН-A1260I) Диапазон

Препаративный насос Agilent 1260 Infinity (ПН-A1260I) — Скорость потока

(ПН-A1260I) Воспроизводимость потока, RSD

(ПН-A1260I) Диапазон давлений

(ПН-A1260I) Диапазон

Стандартный дегазатор Agilent 1260 Infinity (СТД-A1260I) — Скорость потока

(СТД-A1260I) Количество каналов

(СТД-A1260I) Внутренний объём

Микродегазатор Agilent 1260 Infinity (М-A1260I) — Скорость потока

(М-A1260I) Количество каналов

(М-A1260I) Внутренний объём

Термостат колонок (ТК) — Температурный диапазон

(ТК) Кол-во колонок

(ТК) Точность задания температуры

(ТК) Зоны нагрева

(ТК) Время нагрева до 40 °С

(ТК) Время охлаждения с 40 °С до 20 °С

(ТК) Стабильность температуры

Автосамплер (АС) — Система автоматического ввода пробы с возможностью дозирования пробы

(АС) Ёмкость загрузки трея

(АС) Воспроизводимость

(АС) Программируемое количество инжекций пробы из одной виалы

(АС) Точность инжекции при стандартных условиях

(АС) Время цикла инжекции

Флуоресцентный детектор (ФД) — Источник света

(ФД) Частота импульсов

(ФД) Монохроматоры

(ФД) Регистрация спектра

(ФД) Шаг спектра

(ФД) Воспроизводимость длин волн

(ФД) Точность длин волн

(ФД) Частота сбора данных

(ФД) Проточные ячейки

Многоволновой детектор Agilent 1260 Infinity (МД-A1260I) — Тип детектора

(МД-A1260I) Источник света

(МД-A1260I) Количество сигналов

(МД-A1260I) Частота сбора данных

(МД-A1260I) Шум

(МД-A1260I) Дрейф

(МД-A1260I) Линейность

(МД-A1260I) Диапазон длин волн

(МД-A1260I)Точность длин волн

(МД-A1260I) Ширина щели

(МД-A1260I) Ширина диода

(МД-A1260I) Проточные ячейки

Рефрактометрический детектор Agilent 1260 Infinity (РД-A1260I) — Частота сбора данных

(РД-A1260I) Шум

(РД-A1260I) Дрейф

(РД-A1260I) Диапазон коэффициента преломления

(РД-A1260I) Диапазон температур

(РД-A1260I) Диапазон

Детектор с перестраиваемой длиной волны Agilent 1260 Infinity (ДПДВ-A1260I) — Тип детектора

(ДПДВ-A1260I) Источник света

(ДПДВ-A1260I) Количество сигналов

(ДПДВ-A1260I) Частота сбора данных

(ДПДВ-A1260I) Шум

(ДПДВ-A1260I) Дрейф

(ДПДВ-A1260I) Линейность

(ДПДВ-A1260I) Диапазон длин волн

(ДПДВ-A1260I) Точность длин волн

(ДПДВ-A1260I) Ширина щели

(ДПДВ-A1260I) Ширина диода

(ДПДВ-A1260I) Проточные ячейки

Жидкостный хроматограф Agilent 1260 Infinity

0,05…5,0 мл/мин

0,2…10,0 мл/мин

≤0,07 %

±1 %

до 600, поток до 5 мл/мин до 200, поток до 10 мл/мин до 400, поток до 5 мл/мин (модель VL) до 200, поток до 10 мл/мин (модель VL) бар

<2, обычно <1,3 %

0…95 или 5…100 %

0,2…10,0 мл/мин

≤0,07 %

± 1 %

до 600, поток до 5 мл/мин бар

≤0,07 %

±1 %

до 600, поток до 5 мл/мин до 400, поток до 5 мл/мин (модель VL) бар

<2, обычно <1,3 %

0…100 %

0,2…10,0 мл/мин

<0,2 %

±1 %

до 600, поток до 5 мл/мин до 200, поток до 10 мл/мин бар

<2, обычно <1,3 %

0…95 или 5…100 %

<0,2 %

на стороне низкого давления

0,01…20, 0,01…100 (опция) мкл/мин

≤0,7 %

до 400 бар

<0,2 %

1…8,5 pH

0,01…4, 1…2500 (опция) мл/мин

1…99 %

20…400 бар

<0,2 %

1…8,5 pH

0,001…100 мл/мин

≤0,3 %

20…400 бар

1…12,5 pH

до 10 мл/мин

4 шт

12 на канал мл

до 5 мл/мин

4 шт.

1 на канал мл

от 10 ниже лабораторной до 80 °С

не менее 3 колонок длиной до 30 см шт

±0,5 °С

две независимо нагреваемые зоны с элементами Пельтье и датчиком температуры

не более 5 мин

не более 10 мин

± 0,15 °С

объёмом от 0.1…100 мкл с шагом 0,1 мкл, не менее 108 виал на 2 мл

не хуже 0.25% RSD в интервале ввода пробы 5…40 мкл не хуже 0.7% RSD в интервале ввода пробы 1…2 мкл не хуже 1.5% RSD в интервале ввода пробы 0,5…1 мкл

не менее 99 инжекций

не менее 1% (10 мкл)

не более 21 с

0,25 сР

ксеноновая лампа

296 Гц

Вогнутая голографическая решетка, диапазон 200…1200 нм, полоса пропускания 20 нм

Спектры возбуждение или излучения, скорость сканирования 28 мс на точку Например, 0,6 с на спектр 200…400 нм с шагом 10 нм

1…20 нм

±0,2 нм

<±3 нм

74 Гц

стандартная: 8 мкл, до 20 бар полумикро: 4 мкл, до 20 бар биоинертная: 8 мкл, до 20 бар

диодная матрица с 1024 диодами

дейтериевая и вольфрамовая лампы

8 шт

80 Гц

<±0,6×10^-5 при 254 нм и щели 4 нм ед.погл./см

<0,9×10^-3 при 254 нм ед.погл./ч

>2 при 265 нм ед.погл.

190…950 нм

<±1 нм

1, 2, 4, 8, 16 (программируемая) нм

около 1 нм

стандартная: 10 мм, 13,0 мкл, до 120 бар полумикро: 6 мм, 5,0 мкл, до 120 бар микро: 3 мм, 2,0 мкл, до 400 бар полунано: 10 мм, 0,5 мкл, до 50 бар нано: 6 мм, 80 нл, до 50 бар высокого давления: 6 мм, 1,7 мкл, до 400 бар препаративные: 3 мм, 0,3 мм, 0,06

37 Гц

±2,5×10^-9 ед.прелом

<200×10-9 ед.прелом./ч

1,00…1,75

температура окружающей среды +5…55 °C

2,3…9,5 pH

двухлучевой фотометр

дейтериевая лампа

1 шт

80 Гц

<±0,25×10^-5 при 230 нм ед.погл./см

<1×10-4 при 230 нм ед.погл./ч

>2,5 ед.погл.

190…600 нм

<±1 нм

6,5 нм

около 1 нм

стандартная: 10 мм, 14,0 мкл, до 40 бар полумикро: 6 мм, 5,0 мкл, до 40 бар микро: 3 мм, 2,0 мкл, до 120 бар высокого давления: 10 мм, 14,0 мкл, до 400 бар

Пищевая промышленность

анализ пищевых продуктов

Медицина, фармацевтика

контроль качества лекарственных препаратов

• Жидкостный хроматограф Agilent 1260 Infinity.